Quantify cellular and sub-cellular nanoparticle dose for developing predictive models to support accurate dose-response assessments
EMSL Project ID
44751
Abstract
Nanomaterials represent a new class of materials with unique physical and chemical properties with the now widely acknowledged potential to enable new technological advancements in multiple scientific disciplines, including those crucial to the Laboratory and DOE missions in Energy, National Security and the Environment. For these applications and advancements to be successful, understanding the interaction between nanomaterials and biological systems must be advanced to the point where predictive sciences can be applied to the development and engineering of safer nanoparticles. Supporting the needs of the nation in the environmental molecular sciences has been at the core of EMSL mission. Specifically, the investigation of nanoparticle interactions with biological systems is one of the focus topics under EMSL Science of Interfacial Phenomena Theme. The work also addresses the Biological Interactions and Dynamics Science Theme by studying the cellular response to nanoparticle perturbations. In vitro systems and Air-Liquid interface systems are the two main experimental systems used to study the interaction of nanomaterials and biological systems. In both systems, the processes that govern the transport of the nanoparticles to cells, their uptake and fate in the cells, and then the response of the cells to the particles are of great interest and must be characterized quantitatively. The results must be used to develop quantitative predictive models. Since the pathways and fate of nanomaterials within the cell are going to drive the cellular response and ultimately the impact on human health, we will identify and quantify the degree of particle uptake by the cell and within distinct cellular compartments over time, and correlate these measurements with the response and survival of the cell with the ultimate goal of developing predictive models of particle toxicity or biocompatibility. These studies, funded by the NIH and Battelle Toxicology Initiative, will contribute to the design of safer nanomaterials and exposure guidelines.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Karakoti AS, P Munusamy, KE Hostetler, VK Kodali, SVNT Kuchibhatla, G Orr, JG Pounds, JG Teeguarden, BD Thrall, and DR Baer. 2012. "Preparation and Characterization Challenges to Understanding Environmental and Biological Impacts of Nanoparticles." Surface and Interface Analysis 44(8):881-889. doi:10.1002/sia.5006
Xie Y, NG Williams, A Tolic, WB Chrisler, JG Teeguarden, BL Maddux, JG Pounds, A Laskin, and G Orr. 2012. "Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface." Toxicological Sciences 125(2):450-461. doi:10.1093/toxsci/kfr251