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Soybean Proteomics


EMSL Project ID
48310

Abstract

The USA is one of the world's largest producers of soybean (Glycine max), which is important as a source of animal feed, oil and, more recently, for biodiesel production. In recognition of this importance, the DOE-Joint Genome Institute sequenced the soybean genome and has named this plant among its flagship genomes, which will receive greater research attention. The availability of the genome sequence enables detailed functional genomic analysis of soybean. Over the past few years, we have developed a close and productive collaboration with the PNNL EMSL laboratory. This has resulted in the awarding of two, joint grants from NSF and DOE-BER. In both cases, these projects continue our efforts to use soybean root hair cells as a model for studying single cell plant biology. The current project seeks to extend our studies of the root hair response to infection by Bradyrhizobium japonicum, the soybean nitrogen fixing symbiont. Specifically, we seek to utilize proteomics to investigate the role of epigenetic changes (e.g., histone modification) in controlling gene expression responses to infection. We will also use proteomic and metabolomics approaches to explore the role of the plant innate immunity system as a regulator of bacterial infection. Finally, these data will be stored, analyzed and compared through the resources of the Soybean Knowledgebase (SoyKB), which we developed and maintain. The work proposed relates to the area of Biosystem Design and Dynamics. Specifically, the work relates to "regulation of spatial and temporal parameters of metabolic processes."? Aspects of this proposal also focus on the area of biological compartmentalization and metabolic pathways. Since the work focuses on below ground processes, to a lesser extent, it also relates to the area of terrestrial and subsurface ecosystems.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2014-10-01
End Date
2016-09-30
Status
Closed

Team

Principal Investigator

Gary Stacey
Institution
University of Missouri - Columbia

Co-Investigator(s)

Dong Xu
Institution
University of Missouri - Columbia

Team Members

Beverly Agtuca
Institution
University of Missouri - Columbia

Josef Batek Rios
Institution
University of Missouri - Columbia

Ljiljana Pasa-Tolic
Institution
Environmental Molecular Sciences Laboratory