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Identification of radiation affected histone modifications.


EMSL Project ID
48982

Abstract

Biological systems are exceedingly more complex than defined by the genome due to differential protein
expression and degradation, altered mRNA splicing, and the presence of multiple post-translational
modifications (PTMs) that occur in different cellular contexts. Histone proteins in particular are subject to
multiple PTMs that influence chromatin structure and transcription factor recruitment to regulate
transcription in the short or long (e.g. epigenetic) terms. We hypothesize that histones and their complex
array of modifications may serve as a link between environmental perturbations that activate cell
signaling pathways and alterations in gene and protein expression. In response to a perturbation in the
environment, activated cell signaling pathways alter PTMs, localization, and histone binding affinities of
proteins that write and read the specific histone modifications that regulate gene expression patterns. A
key component of our research plan is to utilize intact protein mass spectrometry to comprehensively
map changes in histone PTMs caused by stress, in this case, exposure to low doses of ionizing
radiation.

Project Details

Start Date
2015-04-20
End Date
2015-09-30
Status
Closed

Team

Principal Investigator

David Stenoien
Institution
Pacific Northwest National Laboratory

Related Publications

Zhou M, L Pasa Tolic, and DL Stenoien. 2017. "Profiling of Histone Post-translational Modifications in Mouse Brain with High Resolution Top Down Mass Spectrometry." Journal of Proteome Research 16(2):599-608. doi:10.1021/acs.jproteome.6b00694