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Transcription initiation in the context of chromatin


EMSL Project ID
50680

Abstract

The genetic information of eukaryotic cells is packaged into dense chromatin, which comes in the form of nucleosome arrays. Access to specific genomic sites requires that nucleosomes are remodeled to give access the replication, transcription and repair machinery. This process of nucleosome repositioning is a fundamental part of the transcription initiation process as the transcription start site where RNA polymerase II binds is blocked prior to gene activation. We seek to understand how the remodeling machinery binds and remodels this nucleosomes in the context of transcription initiation by reconstituting a chromatin remodeler with a modified nucleosome.

Project Details

Start Date
2019-03-15
End Date
2019-12-31
Status
Closed

Team

Principal Investigator

Eva Nogales
Institution
University of California, Berkeley

Team Members

Avinash Patel
Institution
Northwestern University

Craig Yoshioka
Institution
Oregon Health & Science University

Related Publications

Patel A., C.M. Moore, B.J. Greber, J. Luo, S.A. Zukin, J. Ranish, and E. Nogales. 2019. "Architecture of the Chromatin Remodeler RSC and Insights into its Nucleosome Engagement." eLife 8. doi:10.7554/eLife.54449