Structure of the first purified manganese oxidase Mnx
EMSL Project ID
51307
Abstract
This project aims to obtain high-resolution atomic structure of the first purified bacterial manganese-oxidizing complex, Mnx. This complex, containing the novel multicopper oxidase MnxG enzyme, has opened a new chapter in bioinorganic chemistry and provided the first opportunity for studying the molecular mechanisms by which bacteria catalyze Mn(II) oxidation with subsequent formation of MnO2 minerals in nature. Cryo-EM studies of the Mnx structure will offer the essential protein structural information to complete the mechanistic view of bacterial manganese biomineralization at the molecular level, expanding our knowledge of an important class of multicopper oxidase enzymes, their role in environmental processes, and the potential for adapting biomineral MnO2 for technological applications. It will also lead to unraveling the biochemical mechanism of bacterial manganese oxidation and bring us closer to understanding the role of microbes in the biogeochemical cycling of manganese.
Project Details
Start Date
2020-03-15
End Date
2021-03-17
Status
Closed
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