COVID-19: Characterization of Respiratory Droplets
EMSL Project ID
60174
Abstract
The COVID-19 pandemic has emphasized the importance of understanding how well viruses survive in the air. SARS-CoV-2 and other airborne pathogens are carried in respiratory droplets consisting of a complex mixture of salts, proteins, lipids, and other components. As droplets evaporate in ambient air, they are subject to phase separation, crystallization, and other changes. Characterizing the physicochemical composition of evaporating respiratory droplets is crucial for understanding the chemical microenvironment to which pathogens are exposed because it modulates their viability. The goal of this limited-scope project is to explore the composition of respiratory droplet residue quantitatively at nanometer-scale spatial resolution. Specifically, we will quantify elemental ratios and their spatial distribution as a function of original droplet size using computer-controlled scanning electron microscopy with energy-dispersive X-ray spectroscopy (CCSEM/EDX) equipped in an environmental scanning electron microscope and infrared scattering scanning near-field optical microscopy (IR s-SNOM). Results will include chemically specific size distributions and spatial distribution of elemental ratios. We expect the elemental ratios to be spatially heterogeneous and to fall in a similar range across different droplet sizes. This project will build scientific capacity and lead to future collaborative efforts between PNNL and Virginia Tech to study bioaerosols.
Project Details
Project type
Limited Scope
Start Date
2021-09-30
End Date
2022-06-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members