Bruce Arey

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Project type

Grand Challenge

As a part of Grand Challenges in Membrane biology, Synechocystis sp. will be investigated in regards of cell morphology and elemental analysis of minerals associated with their outer and inner…

Site-Specific Sample Preparation of Surfaces and Buried Interfaces
for TEM and Atom Probe Analysis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Larry Thomas

Project type

Capability Research

Technique development of methods for preparing cross-sectional TEM samples and atom-probe needle samples with the new dual-beam FIB instrument is proposed. Prior experience in FIB sample preparation…

Role of Microenvironments and Transition Zones in Field scale microbial ecology and biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Biomineralization and Biosequestration Mechanisms at Life's Upper Temperatures: A Reappraisal using Novel CryoEM Techniques

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

Surface Characterization of Calcium Phosphates

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Exploratory Research

Determination of calcium phosphate phases are important in many biomineralization processes from nucleation to growth to inhibition. The factors controlling phase in materials development are…

Characterization of tungsten carbide synthesized by controlled template method

Lead Institution

Ulsan National Institute of Science and Technology

Principal Investigator

Ja Hun Kwak

Project type

Exploratory Research

High purity tungsten carbide materials with high surface area and porosity can provide technically and economically attractive alternates for platinum in an electrocatalysis. We are developing novel…

Three-Dimensional Structural Analysis of Altered Micas by Ion Beam/Scanning Electron Microscopy (FIB/SEM Dual Beam), µ-X-ray Diffraction, and Transmission Electron Microscopy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Bruce Arey

Micaceous minerals such as biotite [K(Mg,Fe)3AlSi¬O10(F,OH)2] and muscovite [KAl2(AlSi3O10)(F,OH)2] are ubiquitous components of detrital sediments, including the soils at the Hanford Site in…

Role of Microenvironments and Transition Zones in Field Scale Microbial Ecology and Biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Limited Scope

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

Structure and property tailoring of metal nanoparticles dispersed in dielectrics by high energy ion implantation

Lead Institution

University of Tennessee

Principal Investigator

Gerd Duscher

Project type

Exploratory Research

High energy ion beam will be used to tailor structure of metal nanoparticles dispersed in dielectric medium. The typical examples are Au, Fe, Co, and Cr implanted into SrTiO3, MgO, TiO2. The…

Ecophysiological Investigation of Cyanobacteria using Controlled Cultivation

Lead Institution

J. Craig Venter Institute

Principal Investigator

Yuri Gorby

Project type

Grand Challenge

This project targets the development and operation of a fully instrumented photobioreactor that will serve to cultivate Cyanobacteria under well defined and controlled conditions. This capability…

In-situ electron microscopy and spectroscopy studies of interfaces in advanced Li-ion batteries under dynamic operation conditions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Chongmin Wang

Repeated charging and discharging of the Li-ion battery induces microstructural evolutions both at the interface between the electrolyte and the electrode and within the electrode (active materials)…

Correlative High-Resolution Imaging and Spectroscopy to Characterize the Structure and Biogeochemical Function of Microbial Biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Exploratory Research

This proposal leverages knowledge and experience gained from DOE-funded research to elucidate the mechanisms involved in dissimilatory metal reduction, as well as, microscale imaging and analytical…

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical cycling, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

Mesopore uniformity and hydrothermal stability of silica thin films for high permeabilty applications

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Xiaohong Li

Project type

Exploratory Research

Thin film OMSs were prepared using several organic templates that provide a range of ordering in the films. Results indicate that ordered mesoporous domains within the film promote uniformly thicker…

SEM-EDS Elemental Mapping of Weymouth Soils

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirk Cantrell

Project type

Exploratory Research

During operation of the former Weymouth phosphate fertilizer plant, sulfuric acid was produced to dissolve phosphate ore. The sulfuric acid was produced from Fe sulfide ore which was composed…

Functional Materials for Separation and Sensing

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jay Grate

Project type

Large-Scale EMSL Research

A large number of mission areas of the US. DOE demand functional materials that will meet the operational requirements of targeted applications. Indeed, innovative solutions to pressing national…

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Use of a Focused Ion Beam for Support of LDRD on Subsampling Radioactive Materials

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Dallas Reilly

PNNL boasts state-of-the-art research capabilities, which are often underutilized on highly radioactive samples due to non-dispersion requirements and limits on sample quantity that can enter…

Evaporative Co-deposition of Fluoride Materials

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Dean Matson

Project type

Exploratory Research

Electron-beam evaporation will be used to co-deposit calcium fluoride and europium fluoride in varying proportions for application in the formation of scintillator films. The project will involve…

Determination of Cr and O diffusion coefficients in (Co,Mn)3O4 coatings for IT-SOFC Steel Interconnects

Lead Institution

Montana State University

Principal Investigator

Richard Smith

Project type

Large-Scale EMSL Research

The long term performance and performance stability of the intermediate temperature solid oxide fuel cells (IT-SOFC) are closely connected to the corrosion resistance and thermal stability of the…

Advanced microstructural analysis of environmental degradation using site-specific transmission electron microscopy and atom-probe tomography

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Daniel Schreiber

Project type

Exploratory Research

Environmental degradation of Ni-based alloys continues to be a major problem in high-temperature water environments such as those found in nuclear reactors and steam generators. Despite several…

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

Routine SEM analysis of lysed Bacillus spores

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Cindy Bruckner-Lea

Project type

Exploratory Research

The research included in this proposal involves the routine SEM analysis of Bacillus thuringiensis and Bacillus atrophaeus spores. SEM analysis will allow us to determine the extent of physical…

Microscopic analysis of carbon-based nanomaterials

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Yongsoon Shin

Project type

Exploratory Research

We are synthesizing nanostructured carbon-based materials from different carbohydrate precursors, such as glucose, fructose, and their derivatives. Functional carbon spheres with core-shell…

Linking Microscopic to Macroscopic Adsorption/Desorption Behavior for Uranium on Environmental Materials

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kevin Rosso

Project type

Large-Scale EMSL Research

This Science Theme proposal requests EMSL access for a subtask on an existing funded project at PNNL. The task is focused on determination of molecular-scale adsorption, desorption, and…

Volumetric Density of Alpha-recoil Tracks in Uranium Oxide by TEM

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Theva Thevuthasan

Considerable effort has been and is being expended to explore various avenues related to nuclear forensics, whether they are for environmental signatures, remediation, or proliferation detection and…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

EMSL Science Overview

Bruce Arey

Projects

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Site-Specific Sample Preparation of Surfaces and Buried Interfaces
for TEM and Atom Probe Analysis

Role of Microenvironments and Transition Zones in Field scale microbial ecology and biogeochemistry

Biomineralization and Biosequestration Mechanisms at Life's Upper Temperatures: A Reappraisal using Novel CryoEM Techniques

Surface Characterization of Calcium Phosphates

Characterization of tungsten carbide synthesized by controlled template method

Three-Dimensional Structural Analysis of Altered Micas by Ion Beam/Scanning Electron Microscopy (FIB/SEM Dual Beam), µ-X-ray Diffraction, and Transmission Electron Microscopy

Role of Microenvironments and Transition Zones in Field Scale Microbial Ecology and Biogeochemistry

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Structure and property tailoring of metal nanoparticles dispersed in dielectrics by high energy ion implantation

Ecophysiological Investigation of Cyanobacteria using Controlled Cultivation

In-situ electron microscopy and spectroscopy studies of interfaces in advanced Li-ion batteries under dynamic operation conditions

Correlative High-Resolution Imaging and Spectroscopy to Characterize the Structure and Biogeochemical Function of Microbial Biofilms

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Mesopore uniformity and hydrothermal stability of silica thin films for high permeabilty applications

SEM-EDS Elemental Mapping of Weymouth Soils

Functional Materials for Separation and Sensing

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Use of a Focused Ion Beam for Support of LDRD on Subsampling Radioactive Materials

Evaporative Co-deposition of Fluoride Materials

Determination of Cr and O diffusion coefficients in (Co,Mn)3O4 coatings for IT-SOFC Steel Interconnects

Advanced microstructural analysis of environmental degradation using site-specific transmission electron microscopy and atom-probe tomography

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Routine SEM analysis of lysed Bacillus spores

Microscopic analysis of carbon-based nanomaterials

Linking Microscopic to Macroscopic Adsorption/Desorption Behavior for Uranium on Environmental Materials

Volumetric Density of Alpha-recoil Tracks in Uranium Oxide by TEM

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone