Reactive and Non-Reactive Deposition of Hyperthermal (1-100 eV) Peptide Ions Surfaces (J Laskin's joint BES/LDRD, PNNL Scope #49904)
EMSL Project ID
16731
Abstract
Interaction of ions with surfaces is an area of active fundamental research in surface science relevant to a broad range of other scientific disciplines such as materials science, mass spectrometry, imaging and spectroscopy. The proposed research will specifically focus on reactive and non-reactive deposition of hyperthermal (1-100 eV) peptide ions on organic surfaces under ultrahigh vacuum conditions. Interaction of ions with surfaces is an area of active fundamental research in surface science relevant to a broad range of other scientific disciplines such as materials science, mass spectrometry, imaging and spectroscopy. Two major processes are dominant for low-energy ion-surface collisions: reactive and non-reactive scattering of ions and ion loss on the surface as a result of neutralization or SL of projectile ions. SL and a related process of reactive landing (RL) of ions, in which ion is covalently attached to the surface, are unique processes that occur during collisions of low-energy ions with self-assembled monolayer (SAM) surfaces. Our recent studies on fundamental aspects of SL of peptide ions provide solid support for this work.. Physical deposition of large ions on surfaces will be studied using a unique Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) developed in our laboratory. Chemical modification of surfaces by biomolecular ions will be explored with an objective to develop a novel approach for preparation of peptide arrays using soft-landing (SL) of mass-selected peptide ions. Modified self-assembled monolayer surfaces will be prepared using a novel ion deposition chamber developed by us in collaboration with Jim Cowin's group (FSD). Physical and chemical characterization of surfaces following SL or RL is crucial for development of a robust analytical technique relevant for biomaterial applications. Surfaces will be characterized using a range of surface characterization facilities at EMSL including surface probe microscopy (AFM/STM/EFM), secondary ion mass spectrometry (TOF-SIMS), polarization modulated infrared reflection spectroscopy (PM-IRRAS). Selected samples will be examined using surface-enhanced raman scattering (SERS) in collaboration with Prof. Jeanne Pemberton (U Arizona).
Exposure of surfaces to ions of known mass and composition presents a highly specific approach for surface modification. This will allow us to prepare surfaces with very high purity, reduce the amount of material required for surface modification and develop new technology relevant for a variety of applications in material science and biotechnology. In addition, we will develop approaches for preparation and characterization of conformationally-selected peptide arrays.
Project Details
Project type
Exploratory Research
Start Date
2005-11-01
End Date
2007-03-19
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Laskin J, P Wang, O Hadjar, JH Futrell, J Alvarez, and RG Cooks. 2007. "Charge Retention by Peptide Ions Soft-Landed onto Self-Assembled Monolayer Surfaces." International Journal of Mass Spectrometry 265(1):237-243.
Wang P, O Hadjar, and J Laskin. 2007. "Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions." Journal of the American Chemical Society 129:8682-8683.