Structural Genomics of Eukaryotic Model Organisms
EMSL Project ID
2610
Abstract
The Northeast Structural Genomics Consortium is a NIH-funded pilot project aimed at evaluating the feasibility, costs, economies of scale, and value of structural proteomics. The primary goal of our pilot project is to develop integrated technologies for high-throughput (htp) protein production and 3D structure determination. Our NESG Consortium has the particular focus of evaluating the role of protein NMR spectroscopy in future Structural Proteomics Centers to be funded by NIH. Our targets include members of large protein families for which no 3D structural information is available. We prioritize protein families with representatives in human and higher eukaryotic genomes because of the important role of these proteins in human health and model organism biology. To date, the NESG Consortium has submitted 35 new protein structures to the PDB. On average, each of these structures allows for homology modeling of ~ 100 protein structures with previously unknown folds (~ 100 modeled structures / structure). We rely heavily on distributed NMR data collection facilities, particularly those at Pacific Northwest National Laboratories. In particular, our center is now competing for a large expansion of budget. This evaluation will be based in part on a site review in Feb 2003. Access to high field NMR time at PNNL is particularly critical in the next 6 month period, during which the consortium as a whole plans to complete and submit 18 - 20 new protein NMR structures to the PDB in order to ensure a positive Feb 2003 review.
Project Details
Project type
Capability Research
Start Date
2002-12-01
End Date
2003-10-23
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Yin C, JM Aramini, LC Ma, JR Cort, GVT Swapna, RM Krug, and G Montelione. 2011. "Backbone and Ile-?1, Leu, Val Methyl 1H, 13C and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein." Biomolecular NMR Assignments 5(2):215-219. doi:10.1007/s12104-011-9303-8