Structural Proteomics: annotating the genome using 3D structure
EMSL Project ID
3333
Abstract
We are applying for NMR spectrometer time at EMSL as a continuation of our structural proteomics project and to study two p53 interacting proteins. Structure of these proteins in complex with p53 will provide insight into the p53 DNA-damage response pathway. Structural proteomics is based on the premise that a protein's biochemical function is often dictated by its 3 dimensional shape. Genome sequence of several organisms have shown that a large percentage of the genome encode for proteins which has no sequence homology to proteins of known function (currently refered to as hypothetical proteins). We hope the structures of these hypothetical proteins would provide some clues as to their function which can then be verified by other biochemical means. To date, our collaboration with Dr. Kennedy?s laboratory had successfully solved 12 structures, 6 of which we were able to deduce the protein?s function.
Project Details
Project type
Capability Research
Start Date
2003-04-12
End Date
2004-04-09
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Bin Wu, Jonathan Lukin, Adelinda Yee, Alexander Lemak, Anthony Semesi, Theresa A. Ramelot, Michael A.Kennedy and Cheryl H. Arrowsmith. 2008. "Solution structure of ribosomal protein L40E, a unique C4 zinc finger protein encoded by archaeon Sulfolobus solfataricus." Protein Sci. 2008 17: 589-596; ;