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Microscopic Fluoresence Imaging of Cellulose Degradation in Microfluidic Devices


EMSL Project ID
39966

Abstract

The goals of this project are to (1) test combinations of fluorescent tag, cellulose form, dye-cellulose conjugation method, and microscopy modalities to demonstrate, for the first time, the ability to image enzymatic removal of fluorescently labeled cellulose from a surface at the micron scale with high analytical precision, and (2) in outyears, use this capability to conduct microbial community experiments in microfluidic porous media models, for the purpose of elucidating the effects of environmental constraints and microbial community interactions on the dynamics of cellulose degradation and CO2 production. We request the unique EMSL fluorescence microscope resources (two-photon confocal and single molecule including STORM) and microfabrication laboratory resources. In outyears, we plan to request transcriptomics analyses on very small samples of microbial communities (10^5 - 10^6 cells) derived from specific regions of the microfluidic porous media models.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2010-10-01
End Date
2013-09-30
Status
Closed

Team

Principal Investigator

Jay Grate
Institution
Pacific Northwest National Laboratory

Team Members

Emily AE Leist
Institution
Pacific Northwest National Laboratory

Andreas Vasdekis
Institution
University of Idaho

Ryan Kelly
Institution
Brigham Young University

Fred Brockman
Institution
Pacific Northwest National Laboratory

Related Publications

Grate JW, C Zhang, TW Wietsma, MG Warner, NC Anheier, Jr, BE Bernacki, G Orr, and M Oostrom. 2010. "A note on the visualization of wetting film structures and a nonwetting immiscible fluid in a pore network micromodel using a solvatochromic dye ." Water Resources Research 46:W11602. doi:10.1029/2010WR009419
Grate JW, RT Kelly, JD Suter, and NC Anheier, Jr. 2012. "Silicon-on-glass pore network micromodels with oxygen-sensing fluorophore films for chemical imaging and defined spatial structure ." Lab on a Chip 12(22):4796-4801. doi:10.1039/C2LC40776K