Microscopic Fluoresence Imaging of Cellulose Degradation in Microfluidic Devices
EMSL Project ID
39966
Abstract
The goals of this project are to (1) test combinations of fluorescent tag, cellulose form, dye-cellulose conjugation method, and microscopy modalities to demonstrate, for the first time, the ability to image enzymatic removal of fluorescently labeled cellulose from a surface at the micron scale with high analytical precision, and (2) in outyears, use this capability to conduct microbial community experiments in microfluidic porous media models, for the purpose of elucidating the effects of environmental constraints and microbial community interactions on the dynamics of cellulose degradation and CO2 production. We request the unique EMSL fluorescence microscope resources (two-photon confocal and single molecule including STORM) and microfabrication laboratory resources. In outyears, we plan to request transcriptomics analyses on very small samples of microbial communities (10^5 - 10^6 cells) derived from specific regions of the microfluidic porous media models.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2010-10-01
End Date
2013-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Grate JW, C Zhang, TW Wietsma, MG Warner, NC Anheier, Jr, BE Bernacki, G Orr, and M Oostrom. 2010. "A note on the visualization of wetting film structures and a nonwetting immiscible fluid in a pore network micromodel using a solvatochromic dye ." Water Resources Research 46:W11602. doi:10.1029/2010WR009419
Grate JW, RT Kelly, JD Suter, and NC Anheier, Jr. 2012. "Silicon-on-glass pore network micromodels with oxygen-sensing fluorophore films for chemical imaging and defined spatial structure ." Lab on a Chip 12(22):4796-4801. doi:10.1039/C2LC40776K