Skip to main content

An integrated top-down and bottom-up proteomics approach to characterize antibodies


EMSL Project ID
48070

Abstract

We describe the application of a new antibody based biomarker discovery approach by mass spectrometry. We previously investigated this approach, and by using different model systems evidence was obtained that it is feasible to identify disease-specific antigen-binding sequences in antibody samples. For a more sensitive, comprehensive and reliable identification of antibody fragments; we will implement new strategies as well as develop novel analytical tools. Thus, this project aims to produce a new and broadly applicable diagnostic tool for the clinical environment. The main focus of the work at the PNNL facilities for this project will be the use of advanced mass spectrometry techniques (top-down) to study larger fragments of immunoglobulins. These techniques are aimed at the unraveling the complete primary structures of disease associated immunoglobulins which can potentially be used as therapeutics and to identify autoimmune and tumor associated antigens.

Project Details

Project type
Limited Scope
Start Date
2013-06-17
End Date
2013-08-17
Status
Closed

Team

Principal Investigator

Leendert Dekker
Institution
Erasmus University Medical Center, Rotterdam

Co-Investigator(s)

Theo Luider
Institution
Erasmus University Medical Center, Rotterdam

Team Members

Martijn vanDuijn
Institution
Erasmus University Medical Center, Rotterdam

Related Publications

Dekker LJ, S Wu, MM vanDuijn, N Tolic, C Stingl, R Zhao, TN Luider, and L Pasa-Tolic. 2014. "An integrated top-down and bottom-up proteomic approach to characterize the antigen binding fragment of antibodies." Proteomics 14(10):1239-1248. doi:10.1002/pmic.201300366
Liu X, LJ Dekker, S Wu, MM vanDuijn, TN Luider, N Tolic, Q Kou, M Dvorkin, S Alexandrova, K Vyatkina, L Pasa-Tolic, and PA Pevzner. 2014. "De Novo Protein Sequencing by Combining Top-Down and Bottom-Up Tandem Mass Spectra." PNNL-SA-103863, Pacific Northwest National Laboratory, Richland, WA. [Unpublished]