Hybrid Microchip/Capillary Electrophoresis Platform for Rapid, Ultrasensitive Bioanalysis
EMSL Project ID
48204
Abstract
Capillary electrophoresis (CE) is a powerful analytical separation technique that is widely used for proteomics, pharmaceutical, and biotechnology applications1-3. We propose to develop a rapid, ultrasensitive, and quantitative chemical analysis platform based on CE coupled with electrospray ionization mass spectrometry (ESI-MS) using a novel microfluidic sample injector. The injector was developed at PNNL previously4-6 as part of an integrated microfluidic device and shown to enable fast, repeatable and unbiased injections. However, the elastomeric device material was not suitable for high resolution separations in the microchip itself. In this work, we will interface the chip-based injector with fused silica capillary separation columns to combine the benefits of the injector with the high separation efficiency and straightforward coupling to ESI-MS enabled by capillaries.
Project Details
Start Date
2013-11-25
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)