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Hybrid Microchip/Capillary Electrophoresis Platform for Rapid, Ultrasensitive Bioanalysis


EMSL Project ID
48204

Abstract

Capillary electrophoresis (CE) is a powerful analytical separation technique that is widely used for proteomics, pharmaceutical, and biotechnology applications1-3. We propose to develop a rapid, ultrasensitive, and quantitative chemical analysis platform based on CE coupled with electrospray ionization mass spectrometry (ESI-MS) using a novel microfluidic sample injector. The injector was developed at PNNL previously4-6 as part of an integrated microfluidic device and shown to enable fast, repeatable and unbiased injections. However, the elastomeric device material was not suitable for high resolution separations in the microchip itself. In this work, we will interface the chip-based injector with fused silica capillary separation columns to combine the benefits of the injector with the high separation efficiency and straightforward coupling to ESI-MS enabled by capillaries.

Project Details

Start Date
2013-11-25
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Keqi Tang
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

Ryan Kelly
Institution
Brigham Young University

Related Publications

Cong Y, SJ Rausch, T Geng, SR Jambovane, and RT Kelly. 2014. "PNEUMATIC MICROVALVE FOR ELECTROKINETIC SAMPLE PRECONCENTRATION AND CAPILLARY ELECTROPHORESIS INJECTION." In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 2560-2561. Chemical and Biological Microsystems Society, San Diego, CA.
Cong Y, S Katipamula, T Geng, SA Prost, K Tang, and RT Kelly. 2015. "Electrokinetic sample preconcentration and hydrodynamic sample injection for microchip electrophoresis using a pneumatic microvalve." ELECTROPHORESIS doi:10.1002/elps.201500286
Kelly RT, C Wang, SJ Rausch, CS Lee, and K Tang. 2014. "Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries." Analytical Chemistry 86(13):6723-6729. doi:10.1021/ac501910p