Metabolic Flux Coupling in Microbial Consortia: Demystifying Metabolic Exchanges across Species
EMSL Project ID
49356
Abstract
We propose to employ EMSL's state-of-the-art metabolomics capabilities to gain a predictive understanding of how compartmentalized metabolic reactions are coordinated across carbon and energy exchanges in microbial communities. We seek to quantitatively determine and predict the flux of metabolites through microbes connected by carbon exchange. To accomplish this, we will constrain our investigation to model microbial consortia composed of cross-feeding heterotrophic bacteria that can be cultivated on defined media, have sequence/annotated genomes and pre-constructed genome-scale metabolic network models. This study will employ an integrative theoretical and experimentation approaches to capitalize upon our team's expertise in advanced microbial co-cultivation, metabolic modelling in combination with EMSL's emerging expertise in 13C Metabolic Flux Analysis. This proposed collaboration with EMSL will enable a broader team scientists, working under a Scientific Focus Area (PNNL's FSFA) sponsored by DOE Office of Science, to learn fundamental biological principles of microbial interaction that control carbon transformation at the community scale. With EMSL's help, we expect the success of this project to be highly impactful as it aims to make a considerable advancement over the current state of this science by integrating 13C-labled metabolic flux analysis and community scale metabolic simulations to quantify and predict the flux of carbon through interacting species in a microbial community.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2016-10-01
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Beck A, HC Bernstein, and R Carlson. 2017. "Stoichiometric network analysis of cyanobacterial acclimation to photosynthesis-associated stresses identifies heterotrophic niches." Processes 5(2):Article No. 32. doi:10.3390/pr5020032
Bernstein H C,McClure R S,Thiel V ,Sadler N C,Kim YM ,Chrisler W B,Hill E A,Bryant D A,Romine M F,Jansson J K,Fredrickson J K,Beliaev A S 2017. "Indirect interspecies regulation; transcriptional and physiological responses of a cyanobacterium to heterotrophic partnership" mSystems 2(2):Article No. e00181-16. 10.1128/mSystems.00181-16
Brislawn C.J., E.B. Graham, K.L. Dana, P.D. Ilhardt, S.J. Fansler, W.B. Chrisler, and J.B. Cliff, et al. 2019. "Forfeiting the priority effect: turnover defines biofilm community succession." The ISME Journal 13, no. 7:1865-1877. PNNL-SA-133067. doi:10.1038/s41396-019-0396-x
Dautel S.E., N.E. Khan, K.R. Brandvold, C.J. Brislawn, J.R. Hutchison, K.K. Weitz, and H.M. Heyman, et al. 2018. "Lactobacillus acidophilus disrupts collaborative multispecies bile acid metabolism." bioRxiv. PNNL-SA-134119. doi:10.1101/296020.
Hill EA, WB Chrisler, AS Beliaev, and HC Bernstein. 2017. "A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks." Bioresource Technology 228:250-256. doi:10.1016/j.biortech.2016.12.111
Khan NE, Y Maezato, RS McClure, CJ Brislawn, JM Mobberley, NG Isern, WB Chrisler, LM Markillie, BM Barney, HS Song, WC Nelson, and HC Bernstein. 2018. "Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture." Scientific Reports 8:297. doi:10.1038/s41598-017-18630-1