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PRedicting ecosystem Resilience through Multiscale and Innovative Science (PREMIS) Initiative: Molecular Phenotyping of Plants & Microbes (MolPheno)


EMSL Project ID
50100

Abstract

The MolPheno project will establish molecular (metabolite and protein) profiles from plants exposed to fresh water-replete or -deprived conditions. Metabolite profiling will include tissue-specific total plant metabolite, plant volatile organic compounds (VOC) and root exudate analysis. Plants, including the genomically well-characterized and tractable model grasses Brachypodium distachyon (Brachypoium) and Setaria virids (Setaria); the newly sequenced diazotrophic (nitrogen-fixing) tree Alnus rubra (red alder), and a few select keystone species from terrestrial-aquatic ecosystem (TAE) sites will be sampled during growth under controlled-environment conditions in the EMSL Phytotron, at the managed PNNL field sites in Richland or at the Marine Sciences Laboratory (MSL) in Sequim, or, for TAE keystone species, on location at select TAE sites.

Project Details

Start Date
2017-12-01
End Date
2020-09-30
Status
Closed

Team

Principal Investigator

Kim Hixson
Institution
Pacific Northwest National Laboratory

Team Members

Pubudu Handakumbura
Institution
Environmental Molecular Sciences Laboratory

Christer Jansson
Institution
Environmental Molecular Sciences Laboratory

John Shilling
Institution
Pacific Northwest National Laboratory

Ryan Renslow
Institution
Pacific Northwest National Laboratory

Erin Baker
Institution
North Carolina State University

Related Publications

Handakumbura P., B.A. Stanfill, A. Rivas-Ubach, D.C. Fortin, J.P. Vogel, and G.C. Jansson. 2019. "Metabotyping as a Stopover in Genome-to-Phenome Mapping." Scientific Reports 9. PNNL-SA-135368. doi:10.1038/s41598-019-38483-0