Kim Hixson

Genome-Enabled Characterization of Orphan Receptor-Like Kinases in Plants

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

This research will characterize the functions and clients (i.e., substrates) of receptor-like kinases (RLKs) of Arabidopsis, rice, and soybean. Objective 1 will reveal RLK functions by combing…

Unlocking the molecular basis of plant-pathogen interactions to create resilient bioenergy crops

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

In the winning 2023-DOE BRAVE (Biopreparedness Research Virtual Environment) Proposal (LAB-23-2955), awarded to co-PI Ljiljana Pasa-Tolic (EMSL/PNNL), PI Qun Liu (BNL), and other co-PI:, Clint Magill…

Dynamics of Microbiomes in Space (DynaMoS)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Janet Jansson

Microorganisms were the first colonizers of our planet and they have since evolved over billions of years to utilize the resources that are available to them. Of all of Earth’s habitats, soil is the…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

An Integrated Omics Guided Approach to Lignification and Gravitational Responses: The Final Frontier

Lead Institution

Washington State University

Principal Investigator

Norman Lewis

Integrated “omics” approaches are increasingly urgently needed to robustly pursue incisive hypotheses in biological systems, while concomitantly gaining better understanding of global ramifications…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Dynamics of Microbiomes in Space (DynaMoS)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Janet Jansson

We propose to examine the population dynamics and community interactions of naturally co-adapted soil microbial consortia using multi-omics analysis, correlative molecular networking and metagenomics…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

SyPro Poplar: Improving Poplar Biomass Production under Abiotic Stress Conditions: an
Integrated Omics, Bioinformatics, Synthetic Biology and Genetic Engineering Approach

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

To engineer bioenergy crops that can grow in dry, marginal lands, this project will introduce into poplar trees tolerance to different environmental stresses such as water deficit, high soil salinity…

Integrated Stable Isotope Probing for the Comprehensive Quantitative- Characterization of Biological Dynamics and Elemental Cycling

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mary Lipton

Biological organisms dynamically function in relationship to their environment and neighboring organisms, creating a continual interplay that constitutes the ecological state. Fundamental…

Tracing rhizosphere carbon exchange processes and nutrient interactions

Lead Institution

Michigan State University

Principal Investigator

James Moran

Rhizodeposition represents the largest flux of photosynthetically derived organic matter into soil for most terrestrial systems and, in so doing, serves as a major point of connection between…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Dynamics of Microbiomes in Space (DynaMoS)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Janet Jansson

Microorganisms were the first colonizers of our planet and they have since evolved over billions of years to utilize the resources that are available to them. Of all of Earth’s habitats, soil is the…

Molecular Phenotyping of Brachypodium to Provide Metabolic and Functional Linkages to the Plant-Atmosphere-Soil-Systems (iPASS) Model

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal aims to provide the fundamental molecular characterization necessary to develop a model which ultimately links phenotypic measurements to the atmospheric processes and soil…

Elucidation of the Roles of Diazotrophic EndoPopulus: Endophyte Communities in Promoting Productivity and Resilience of Populus through Systems Biology Approaches (EndoPopulus)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

The plant microbiome, including microbial endophytes living within plants and the epiphytes living on the plant surface, has a profound influence on the productivity and resilience of the host plant…

PhenoAccess: Physiological Phenotyping of Brachypodium Accessions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

Although the effects of elevated CO2 on plant growth, physiology and metabolism has been investigated thoroughly, the underlying integrated organismal, cellular and molecular mechanisms of these…

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Stephanie Thibert

Macromolecular assemblies in cell membrane and organelles are compositionally and structurally complex and their identification and characterization represent a major barrier to understanding…

Consortium for Advanced Sorghum Phenomics (CASP)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

The mission of the proposed project is to accelerate breeding of biomass sorghum to maximize terminal compositional (e.g., lignocellulosic) yield under drought and saline field conditions using novel…

Deep UV laser ablation system for spatial single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

William Chrisler

Laser capture microdissection (LCM) is the key capability for spatially resolved omicss. However, commercially available LCM systems are designed for mammalian tissues and feature UV lasers (330 nm),…

NBACC: Host/pathogen interactions (PNNL Scope # 49488, Lipton's NBACC Host project)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mary Lipton

Determine the host pathogen interactions from Yersinia pestis infected into rat animal models. In collaboration with Walter Reed Army research center, rats will be infected with Yersina pestis and…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

C4 Photosynthesis in Space (C4Space)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Pubudu Handakumbura

C4 plants like maize (Zea mays) and sorghum (Sorghum bicolor) have a more efficient photosynthesis than C3 plants such as wheat (Triticum aestivum) and rice (Oryza sativa) due to a CO2-concentrating…

Developing Calculation Methods to Precisely Measure Proteome Proteins and Their Post-Translational Modifications

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nikola Tolic

In this proposed work we intend to: 1) Make an UStag caluclation algorithm into a high-throughput format that would be user friendly fo rthe general proteome community; 2) Create a human post…

PRedicting ecosystem Resilience through Multiscale and Innovative Science (PREMIS) Initiative: Molecular Phenotyping of Plants & Microbes (MolPheno)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

The MolPheno project will establish molecular (metabolite and protein) profiles from plants exposed to fresh water-replete or -deprived conditions. Metabolite profiling will include tissue-specific…

Dynamics of Microbiomes in Space (DynaMoS)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Janet Jansson

Microorganisms were the first colonizers of our planet and they have since evolved over billions of years to utilize the resources that are available to them. Of all of Earth’s habitats, soil is the…

High-Throughput Proteomics: Applications and Development (PNNL Scope #40601 and #43488)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Gordon Anderson

This project involves the application and development of high-throughput proteomics and quantitative measurement capabilities within biological systems. More so, application measurements will be…

InterCom

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

We intend to identify how molecular interactions at the intercellular level participate in controlling carbon assimilation, allocation and transformation in the plant-microbe-soil system (Fig. 1),…

Identification and quantification of biomarkers of malaria infection.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This application is for the continuation of the currently closed proposal 8211 "Membrane proteins of placental parasites". We have a joint grant proposal between Richard D. Smith and Michal…

SyPro Poplar: Improving Poplar Biomass Production under Abiotic Stress Conditions: an
Integrated Omics, Bioinformatics, Synthetic Biology and Genetic Engineering Approach

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

To engineer bioenergy crops that can grow in dry, marginal lands, this project will introduce into poplar trees tolerance to different environmental stresses such as water deficit, high soil salinity…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Mapping Nutrient Flow through a Plant-Microbe Symbiont System using Stable Isotope Probing under Drought and Salinity Stress

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

Red alder (Alnus rubra) is one of the fastest growing, commercially important, hardwood species for biomass/timber production in North America, and is also involved in a nitrogen-fixation symbiosis…

Rhizosphere Research Campaign: The plant-soil interface - understanding dynamic interactions in the context of environmental change

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

Project type

Research Campaign

The ultimate, long-term goal is to determine what drives source-sink relationships that control the cycling of plant nutrients belowground. To fully appreciate the complexities of this dynamic…

Consortium for Advanced Sorghum Phenomics (CASP)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

The mission of the proposed project is to accelerate breeding of biomass sorghum to maximize terminal compositional (e.g., lignocellulosic) yield under drought and saline field conditions using novel…

Proteome Response to Arogenate Dehydratase Changes in Arabidopsis and Poplar

Lead Institution

Washington State University

Principal Investigator

Norman Lewis

Project type

Exploratory Research

Arogenate dehydratases (ADTs) are a class of isoenzymes involved in the production of phenylalanine (Phe). In addition to its involvement in protein biosynthesis, Phe is the starting metabolite in…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Multi-Omics Evaluation of Tripartite Associations in Red Alder Roots: Integrated Transcriptomics, Proteomics, Metabolomics and Metabolite Imaging of Red Alder Symbiotic Associations in Root Tissue

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal presents the unique opportunity to conduct the first ever integrated multi-omics and imaging evaluation of the Alnus rubra (red alder tree)/Frankia alni (bacteria)/Alpova diplophloeus …

Molecular Phenotyping of Plants & Microbes (MolPheno) for the PRedicting Ecosystem Resilience through Multiscale and Integrative Science (PREMIS) initiative

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal aims to provide the fundamental molecular characterization necessary to develop a model which ultimately links phenotypic measurements to the soil biogeochemistry associated with…

Projects