Genome-Enabled Characterization of Orphan Receptor-Like Kinases in Plants
EMSL Project ID
61095
Abstract
This research will characterize the functions and clients (i.e., substrates) of receptor-like kinases (RLKs) of Arabidopsis, rice, and soybean. Objective 1 will reveal RLK functions by combing quantification of phosphorylation and S-acylation (e.g., palmitoylation) as indicators of involvement of an RLK in biological process. Specifically bottom-up proteomics technology will quantitate total and plasma membrane-enriched proteins during the relatively well-understood Arabidopsis-Psuedomas syringae interaction; across rice internode development with and without drought stress; and in the soybean and rice root responses to conserved putative small secreted peptides. Objective 2 will create peptide libraries for each species and screen them against purified protein kinases to identify clients for novel kinases. Objective 3, will extend experimental results using machine learning to predict plant phosphorylation sites and kinase clients. Proteome data will also be used for molecular network reconstruction and comparative analysis to identify RLK signaling patterns. Objective 4 will take a tiered approach to outreach that melds plant and data science. Teachers recruited for professional development experiences will aid in recruiting Native American high school and undergraduate students to week-long research introduction workshops; then these students will have the opportunity to join others for summer and academic year undergraduate research experiences.
Project Details
Start Date
N/A
End Date
N/A
Status
Accepted
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members