Ryan Renslow

Fundamental processes involved in scale up and their role in extracellular electron transfer

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Large-Scale EMSL Research

Our research group use microbial fuel cells (MFCs) as a tool to study electron transfer processes from biofilms to solid electrodes. Although MFCs have been well studied in the literature,…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Combined nuclear magnetic resonance and electrochemical techniques for the study of electrochemically-active Geobacter biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Project type

Large-Scale EMSL Research

Understanding the ability of some microorganisms to utilize extracellular electron transfer is important for applications in bioremediation and electrochemically-stimulated synthetic biology…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Simultaneous Electrochemical and Nuclear Magnetic Resonance Techniques for the Study of Electrochemically Active Biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

It is now known that some microbial communities, known as electrochemically active biofilms, have the ability to use solid conducting materials as their terminal electron acceptors for respiration…

Pacific Northwest Advanced Compound Identification Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The capability to chemically identify thousands of metabolites and other chemicals in clinical samples will revolutionize the search for environmental, dietary, and metabolic determinants of disease…

Fundamental research at the nano- and microscale to understand how biofilms interact with wounds in the presence of hyperosmotic agents

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

The bactericidal effect of extracellular hyperosmosis has been acknowledged for decades1,2. Nevertheless, it was only recently shown that hyperosmosis from high NaCl concentrations effectively…

Pacific Northwest Advanced Compound Identification Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The capability to chemically identify thousands of metabolites and other chemicals in clinical samples will revolutionize the search for environmental, dietary, and metabolic determinants of disease…

Fundamental research at the nano- and microscale to understand how biofilms interact with wounds in the presence of hyperosmotic agents

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

The bactericidal effect of extracellular hyperosmosis has been acknowledged for decades1,2. Nevertheless, it was only recently shown that hyperosmosis from high NaCl concentrations effectively…

Prediction of response in soil microbiome carbon utilization to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

Project type

FICUS Research

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Investigating Electrochemically Stimulated Biology Using Combined Electrochemical - Nuclear Magnetic Resonance (EC-NMR) Techniques

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Understanding the ability of some microorganisms to utilize extracellular electron transfer is important for applications in bioremediation and electrochemically-stimulated synthetic biology…

Pacific Northwest Advanced Compound Identification Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The capability to chemically identify thousands of metabolites and other chemicals in clinical samples will revolutionize the search for environmental, dietary, and metabolic determinants of disease…

Fundamental research at the nano- and microscale to understand how biofilms interact with wounds in the presence of hyperosmotic agents

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

The bactericidal effect of extracellular hyperosmosis has been acknowledged for decades1,2. Nevertheless, it was only recently shown that hyperosmosis from high NaCl concentrations effectively…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

In situ mesoscale imaging of live biofilm dynamics using SALVI

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Xiao-Ying Yu

We seek to understand how the dynamics of nutrients, metabolites and metals influence microbial processes in biofilm systems. Our approach will use axenic or heterogeneous microbial communities to…

Backfilling the Natural Products Magnetic Resonance Database with DFT Calculated NMR Chemical Shifts

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

The gold standard for unequivocal identification of a novel natural product (NP) or other small molecule is structure elucidation by NMR spectroscopy. Although, over 100,000 natural products have…

Deciphering Microbial Communication Through Metabolites

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

Computationally-driven discovery and identification of small molecules in biological systems

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

In this project we will build the critical tools for an eventual full standards-free (e.g. computation derived identification libraries) and library-free (i.e. de novo) small molecule identification…

In situ monitoring of arginine in living dental biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Dental caries disease is one of the most widespread diseases globally. A hallmark of supragingival microbial communities is the ability to rapidly metabolize carbohydrates, which dramatically reduces…

High-throughput Genome-to-Metabolome Computational Methods for Microbiome Metabolomics and Modeling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Quantum chemical calculations have been used to predict NMR chemical shifts of metabolites; however, to date, these calculations have been limited to small sets of molecules (less than 50). Datasets…

In silico Chemical Library Engine (ISICLE) for Expo/Metabo-lomics and Modeling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan Renslow

Currently, there are no nuclear magnetic resonance or IMS databases specifically built for identifying and quantifying metabolites of relevance to environmental samples in general, or to microbial…

Biofilm reactor development and characterization studies

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Large-Scale EMSL Research

This proposal addresses the development of biofilm-based methods for the economical production of biochemicals and bio-fuels. Biofilms have the potential to solve many of the problems of conventional…

Molecular Phenotyping of Brachypodium to Provide Metabolic and Functional Linkages to the Plant-Atmosphere-Soil-Systems (iPASS) Model

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal aims to provide the fundamental molecular characterization necessary to develop a model which ultimately links phenotypic measurements to the atmospheric processes and soil…

MICROSCALE METABOLIC, REDOX AND ABIOTIC REACTIONS IN HANFORD 300 AREA SUBSURFACE SEDIMENTS

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Exploratory Research

This proposal is the NMR component of a funded DOE/ERSP program project in which we proposed to use the Hanford 300 Area as a site for identifying microscale metabolic, abiotic and redox reactions in…

FSFA: MOSAIC—Metabolic and Spatial Interactions in Communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Errol Robinson

This EMSL user proposal is to support the MOSAIC DOE BER funded FSFA at PNNL. The primary activities involving EMSL are a set of BSSD demonstration tasks focusing on key science questions and related…

Electron Transfer Mechanisms in Biofilms

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Large-Scale EMSL Research

Although interactions of microorganisms with electrodes have been studied in the literature, the mechanisms of electron transfer are still poorly understood. The goal of this proposal is to…

FSFA: MOSAIC -- Metabolic and Spatial Interactions in Communities FY18 proposal

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Errol Robinson

This EMSL user proposal is to support the MOSAIC DOE BER funded FSFA at PNNL. The primary activities involving EMSL are a set of BSSD demonstration tasks focusing on key science questions and related…

Microscale Metabolic, Redox and Abiotic Reactions in Hanford 300 Area Subsurface Sediments

Lead Institution

Washington State University

Principal Investigator

Haluk Beyenal

Project type

Large-Scale EMSL Research

This proposal is the NMR component of a funded DOE/ERSP program project in which we proposed to use the Hanford 300 Area as a site for identifying microscale metabolic, abiotic and redox reactions in…

PRedicting ecosystem Resilience through Multiscale and Innovative Science (PREMIS) Initiative: Molecular Phenotyping of Plants & Microbes (MolPheno)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

The MolPheno project will establish molecular (metabolite and protein) profiles from plants exposed to fresh water-replete or -deprived conditions. Metabolite profiling will include tissue-specific…

Projects