Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.
EMSL Project ID
50630
Abstract
The goal of this research proposal is to establish concrete design principles that define and control efficient proton (H+) transport for [FeFe]-hydrogenase (H2ase). Proteins are able to perform remarkably efficient spatial and temporal delivery of H+s with the use of precisely controlled H+ pathways. These pathways are formed by a series of H+ relays, functional groups capable of accepting and donating H+s. In spite of the broad need for understanding H+ transfer at a molecular level, mechanistic design principles for H+ transport are not well formulated. The proposed work will use a H2ase to understand H+ transport for this enzyme and will contribute a deeper mechanistic understanding for the control of H+-coupled electron transfer that can be expanded to other enzyme systems. Project Details
Start Date
2018-11-26
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members