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Advanced MS instrumentation and methods for functional proteomics


EMSL Project ID
50699

Abstract

This proposal addresses a critical need to continue to upgrade the spectrometer for the 21T FTICR-MS in order to keep this flagship capability on the leading edge of technology. The proposed work builds upon EMSL’s leadership in proteomics and will yield a world-class platform with unmatched capabilities for structural biology and top-down characterization of the functional proteome. We will develop a hybrid Orbitrap-FTICR-MS with advanced tandem mass spectrometry (MS/MS) capabilities to enable top-down and native MS experiments for characterization of functional proteoforms1 and their assembly into molecular machines2 that perform all cellular functions.

Project Details

Start Date
2019-01-25
End Date
2019-09-30
Status
Closed

Team

Principal Investigator

Jared Shaw
Institution
Environmental Molecular Sciences Laboratory

Team Members

Matthias Biel
Institution
Thermo Fisher Scientific

Wendy Sandoval
Institution
Genentech, Inc.

Weijing Liu
Institution
Pacific Northwest National Laboratory

Aivett Bilbao Pena
Institution
Environmental Molecular Sciences Laboratory

Joon-Yong Lee
Institution
Pacific Northwest National Laboratory

Edward Hoegg
Institution
Pacific Northwest National Laboratory

Mowei Zhou
Institution
Environmental Molecular Sciences Laboratory

Related Publications

Shaw J.B., W. Liu, Y.V. Vasil'Ev, C.C. Bracken, N. Malhan, A.L. Guthals, and J.S. Beckman, et al. 2020. "Direct Determination of Antibody Chain Pairing by Top-down and Middle-down Mass Spectrometry using Electron Capture Dissociation and Ultraviolet Photodissociation." Analytical Chemistry 92, no. 1:766-773. PNNL-SA-145157. doi:10.1021/acs.analchem.9b03129
Zhou M., W. Liu, and J.B. Shaw. 2020. "Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry." Analytical Chemistry 92, no. 2:1788-1795. PNNL-SA-145158. doi:10.1021/acs.analchem.9b03469