Advanced MS instrumentation and methods for functional proteomics
EMSL Project ID
50699
Abstract
This proposal addresses a critical need to continue to upgrade the spectrometer for the 21T FTICR-MS in order to keep this flagship capability on the leading edge of technology. The proposed work builds upon EMSL’s leadership in proteomics and will yield a world-class platform with unmatched capabilities for structural biology and top-down characterization of the functional proteome. We will develop a hybrid Orbitrap-FTICR-MS with advanced tandem mass spectrometry (MS/MS) capabilities to enable top-down and native MS experiments for characterization of functional proteoforms1 and their assembly into molecular machines2 that perform all cellular functions.
Project Details
Start Date
2019-01-25
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Shaw J.B., W. Liu, Y.V. Vasil'Ev, C.C. Bracken, N. Malhan, A.L. Guthals, and J.S. Beckman, et al. 2020. "Direct Determination of Antibody Chain Pairing by Top-down and Middle-down Mass Spectrometry using Electron Capture Dissociation and Ultraviolet Photodissociation." Analytical Chemistry 92, no. 1:766-773. PNNL-SA-145157. doi:10.1021/acs.analchem.9b03129
Zhou M., W. Liu, and J.B. Shaw. 2020. "Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry." Analytical Chemistry 92, no. 2:1788-1795. PNNL-SA-145158. doi:10.1021/acs.analchem.9b03469