Mowei Zhou

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tong Zhang

To use PTM data to understand cell signaling and profile molecular phenotypes, PNNL has made substantial strides in advancing phosphoproteome and thiol redox proteome profiling. However, the current…

Super resolution fluorescence imaging of plant receptors

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dehong Hu

This proposal covers work done under a DOE BER funded research project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence…

Proteomic study of Transthyretin Protein

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

The project will establish a scientific partnership between the cardiomyopathy section at OHSU and mass spectrometry facility at PNNL which leverages the strengths of OHSU Cardiomyopathy center …

Statistical Methodology for Characterization of Macromolecular Similarity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

As with any drug, approval of generic versions of macromolecular drugs requires rigorous evaluation of therapeutic equivalence to the reference drug in order to assure similar efficacy and safety…

High throughput and in-depth single-cell proteomics for plant cells

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

In support of the "Single-cell biology" value stream in EMSD/EMSL DigiPhen Motivation, we propose to develop a high throughput and in-depth single-cell proteomics platform. We aim to…

Statistical Methodology for Characterization of Macromolecular Similarity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

As with any drug, approval of generic versions of macromolecular drugs requires rigorous evaluation of therapeutic equivalence to the reference drug in order to assure similar efficacy and safety…

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

We propose to apply an integrated multi-modal structural biology approach to investigate a biosynthetic protein complex in plants that is hypothesized to organize and guide lignin biosynthesis via…

Statistical Methodology for Characterization of Macromolecular Similarity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

As with any drug, approval of generic versions of macromolecular drugs requires rigorous evaluation of therapeutic equivalence to the reference drug in order to assure similar efficacy and safety…

COVID-19: Molecular design and analysis to inform small molecule therapeutics related to COVID-19

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Neeraj Kumar

The multi-laboratory project will apply DOE capabilities to complement resources available in the public and private sector and thereby accelerate discovery of medical therapeutics targeting SARS-COV…

Synthesis and Structural Probing of Protein Networks in Vitamin B6 Biosynthesis.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Irina El Khoury

We propose to develop a multi-modal experimental pipeline, integrating multiple existing and emerging EMSL capabilities, including cell-free protein expression, Native Mass Spectrometry (Native MS),…

Spatially resolved characterization of proteoforms for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Differentiated cells have distinctive patterns of epigenetic marks including various post-translational modifications (PTMs) on histones that may work in concert to control transcriptional programs…

Development of an Integrated Structural Mass Spectrometry Platform for Unveiling the Structure of an Unknown Protein Complex Machinery in Lignin Synthesis

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Multiple distinct Lignin-Forming Complexes (LFC) are proposed to be templates and catalysts that orchestrate lignin synthesis in plants. It is hypothesized that each LFC consists of one or more…

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Stephanie Thibert

Macromolecular assemblies in cell membrane and organelles are compositionally and structurally complex and their identification and characterization represent a major barrier to understanding…

Development of a single-cell top-down proteomics platform for understanding rhizobial infection process

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

We aim to extend EMSL’s leading capability in ultrasensitive/single cell proteomics to measure intact proteins in individual plant cells. We will address major challenges in ultrasensitive top down…

Towards proteome-wide assessment of cellular landscapes

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

Advanced MS instrumentation and methods for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

This proposal addresses a critical need to continue to upgrade the spectrometer for the 21T FTICR-MS in order to keep this flagship capability on the leading edge of technology. The proposed work…

Automating the data analysis workflow for relative quantitation of isomeric proteoforms based on fragment-ion intensities in top-down LC-MS/MS analyses

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Aivett Bilbao Pena

We will develop a software tool for relative quantitation of isomeric proteoforms based on fragment-ion intensities in liquid chromatography-tandem mass spectrometry (LC-MS/MS) data from top-down…

Native Mass Spectrometry Enabled Electron Microscopy for Visual Proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

Develop a soft-landing capability for native protein complexes coupled with electron microscopy (EM) to enable visual proteomics. Sample heterogeneity greatly limits the resolution of 3D structures…

Virtual Proteomics (ViP): Integrative modeling of protein assemblies in a cell with constraints from proteomic data and reconstructed images from cryo-electron tomography

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The objective of the proposed research is to advance the spatial knowledge of higher-order protein assemblies in a cell by integrating improved image reconstruction from cryo-electron tomography and…

Manganese biomineralization by the bacterial protein complex Mnx

Lead Institution

University of Washington

Principal Investigator

Bradley Tebo

Project type

Large-Scale EMSL Research

Manganese-oxide mineral formation by bacterial oxidation of dissolved Mn(II) is an important process in aquatic and soil environments and is a key part of the global Mn cycle. The Mn bio-oxides…

Mechanistic studies of AAP and capsid assembly of AAV vectors

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Adeno-associated viruses (AAV) have become increasingly popular as vectors for human gene therapy. However, the requirement for high vector doses needed to achieve therapeutic efficacy poses a…

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Recent developments of state-of-the-art mass spectrometry (MS) instrumentation at EMSL, including the 21T Fourier Transform Ion Cyclotron Resonance (FTICR) MS, have enabled high resolution and high…

High-throughput native mass spectrometry as experimental validation for in silico drug design

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Enzyme functions can often be modulated via interaction with small molecules. For example, inhibitors are actively sought in the pharmaceutical industry to block protein targets and treat diseases…

Development of in vitro glycosylation toolkit to study eukaryotic glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Irina El Khoury

When working with glycoproteins from native sources, biochemical analysis is often hampered by sample heterogeneity and glycan-postprocessing structural diversity. It is therefore desirable to create…

Advanced 21T FTICR MS instrumentation and methods for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

The 21T FTICR-MS at the EMSL provides extremely high mass accuracy and resolution enabling superior depth of characterization for natural organic matter and intact mass measurement of denatured…

Multiplexed high resolution fluorescence imaging in plants

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

This proposal covers work done under a DOE-funded project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence microscopy platform,…

Next generation microfluidics platform for high throughput single cell proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

The project aims to improve both sensitivity and throughput in single cell proteomics through the development of a microfluidic platform that leverages current technology. A hybrid picowell/nanowell…

COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

The project aims to develop a high throughput and multiplex affinity purification mass spectrometry (AP-MS) assay by seamless integration of protein microarray and nanoscale proteomics. We will…

Characterization of an Expressed Bacterial Mn(II,III)-Oxidizing Multicopper Oxidase Complex and its Mn Oxide Products

Lead Institution

University of Washington

Principal Investigator

Bradley Tebo

Project type

Large-Scale EMSL Research

Microbial Mn(II) oxidation is an important environmental process that links the biogeochemical cycle of Mn with the cycling of C, N, P, and many trace metals and radionuclides. Microbes that oxidize…

Development of a functional proteomics workflow for characterization of glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Glycosylation is a diverse family of post-translational modification (PTM) that affects protein structure/function but is not directly encoded in the genome, and represents significant challenge for…

Projects