Multiplexed high resolution fluorescence imaging in plants
EMSL Project ID
51203
Abstract
This proposal covers work done under a DOE-funded project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence microscopy platform, including the development of fluorescent quantum nanomaterials, which integrates imaging in the visible and near infrared (NIR) regions to enable high resolution multiplexed imaging in plants. The overlapping emission spectra of endogenous plant pigments currently limit the number of fluorophores with different emission colors that can be imaged simultaneously and, therefore, the number of cellular components that can be imaged within a single experiment. Hence, developing fluorescent tags and a microscope system that can image beyond the visible range deep into the NIR would greatly enhance plant imaging capabilities. Extending imaging into the NIR region would support the interrogation of multiple cellular molecular targets simultaneously. In parallel, effort pursued at the University of Maryland Baltimore County will focus on the development of quantum dots (QDs) and polymer dots (Pdots) that emit both at the visible and NIR regions. Pdots encapsulating 2-3 organic dyes emitting in the visible and the NIR regions will be also developed to enable multiplexed imaging using fluorescence barcodes. The dots will be used to tagged putative membrane receptors, identified by proteomics. The application of the new imaging platform will be demonstrated by studying the functional identify of these membrane receptors.
Project Details
Start Date
2019-11-04
End Date
2022-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Connor Riahin, Kushani Mendis, Brandon Busick, Marcin Ptaszek, Mengran Yang, Gary Stacey, Amar Parvate, James E. Evans, Jeremiah Traeger, Dehong Hu, Galya Orr, Zeev Rosenzweig. 2022. "Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing." Sensors 22 (19):7218. 10.3390/s22197218
Riahin C, Meares A, Esemoto NN, Ptaszek M, LaScola M, Pandala N, Lavik E, Yang M, Stacey G, Hu D, Traeger JC, Orr G, Rosenzweig Z. Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging. ACS Applied Materials & Interfaces. 2022;14:20790−801.