Development of a single-cell top-down proteomics platform for understanding rhizobial infection process
EMSL Project ID
50668
Abstract
We aim to extend EMSL’s leading capability in ultrasensitive/single cell proteomics to measure intact proteins in individual plant cells. We will address major challenges in ultrasensitive top down proteomics including: 1) How to reduce protein loss to container surface during sample processing? 2) How to efficiently separate low amount of proteins and identify them by high resolution MS/MS? We will integrate the top-down mass spectrometry capability with our single cell proteomics technology to profile protein post-translational modifications during early nodule development and to better understand rhizobial infection process.
Project Details
Start Date
2019-01-11
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members