Development of a single-cell top-down proteomics platform for understanding rhizobial infection process
EMSL Project ID
50668
Abstract
We aim to extend EMSL’s leading capability in ultrasensitive/single cell proteomics to measure intact proteins in individual plant cells. We will address major challenges in ultrasensitive top down proteomics including: 1) How to reduce protein loss to container surface during sample processing? 2) How to efficiently separate low amount of proteins and identify them by high resolution MS/MS? We will integrate the top-down mass spectrometry capability with our single cell proteomics technology to profile protein post-translational modifications during early nodule development and to better understand rhizobial infection process.
Project Details
Start Date
2019-01-11
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Cong Y., Y. Liang, K. Motamedchaboki, R. Huguet, T. Truong, R. Zhao, and Y. Shen, et al. 2020. "Improved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry." Analytical Chemistry 92, no. 3:2665-2671. PNNL-SA-148385. doi:10.1021/acs.analchem.9b04631
Dou M., C. Tsai, P.D. Piehowski, Y. Wang, T.L. Fillmore, R. Zhao, and R.J. Moore, et al. 2019. "Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples." Analytical Chemistry 91, no. 15:9707-9715. PNNL-SA-141437. doi:10.1021/acs.analchem.9b01248
Zhou M, Uwugiaren N, Williams SM, Moore RJ, Zhao R, Goodlett D, Dapic I, Pa?a-Tolić L, Zhu Y (2020) Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform. Anal Chem 92: 7087?7095.