Development of in vitro glycosylation toolkit to study eukaryotic glycoproteins
EMSL Project ID
51145
Abstract
When working with glycoproteins from native sources, biochemical analysis is often hampered by sample heterogeneity and glycan-postprocessing structural diversity. It is therefore desirable to create a simplified platform in which glycosylation events can be re-created in a controlled environment, thereby permitting the study and engineering of glycoproteins on demand. Such a framework, the in vitro glycosylation toolkit, has been recently developed using a bacterial cell-free platform coupled with bacterial glycotransferases and appropriate glycan precursors1,2. However, an in vitro glycosylation toolkit is not yet available for eukaryotic systems due to lack of a fundamental understanding of the enzymes involved. Through this proposal, we plan to deploy an in-house eukaryotic cell-free platform to synthesize and characterize plant glycotransferases with the goal of engineering glycoproteins that are important for environmental and bioenergy applications.
Project Details
Start Date
2019-10-01
End Date
2021-12-31
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members