Projects

Grand Challenge in Biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Fredrickson

Project type

Grand Challenge

Do multiheme cytochromes associated with the outer membrane of metal reducing bacteria (mtrC in Shewanella oneidensis and omcB in Geobacter sulfurreducens, for example) transfer electrons to Fe(III)…

Thrust Area 1: Characterization of Purified Proteins for Distribution to Grand Challenge Participants (EMSL User Support)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Liang Shi

Project type

Grand Challenge

A facultative anaerobic bacterium, Shewanella oneidensis MR-1 is able to use metal oxides, such as Mn(IV) and Fe(III), as terminal electron acceptors during anaerobic respiration. Since Mn(IV) and…

Thrust 3,4: Molecular Forces in Bacterial Adhesion at the Oxide-Water Interface

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Michael Hochella

Project type

Grand Challenge

1) Influence of Fe(III) Oxide Particle Size on Organism/Oxide Interfacial Contact Area and Electron Transfer Rates. Dr. Hochella’s research group will investigate how the surface properties of…

Thrust Area I: Physiology of metal-reducing strains of Shewanella.

Lead Institution

University of Southern California

Principal Investigator

Kenneth Nealson

Project type

Grand Challenge

A multidisciplinary team of scientists is investigating the mechanisms of electron transfer from metal-reducing bacterial to Fe oxide surface as part of a PNNL/DOE-BER Grand Challenge in…

Thrust 4: Model Hematite Thin Films and Bacterial Iron Reduction (LDRD: Cytochrome and Whole Cell Interactions with Iron Oxides)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Timothy Droubay

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Thrust 4: Surface Enhanced Raman-AFM and Antibody Force Microscopy (LDRD: Cytochrome and Whole Cell Ineteractions with Iron Oxides)

Lead Institution

Bowling Green State University

Principal Investigator

H. Peter Lu

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Thrust 4: Whole Cell and Cytochrome Biological Force Microscopy (LDRD: Cytochrome and Whole Cell Interactions wtih Iron Oxides)

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Ecophysiological Investigation of Cyanobacteria using Controlled Cultivation

Lead Institution

J. Craig Venter Institute

Principal Investigator

Yuri Gorby

Project type

Grand Challenge

This project targets the development and operation of a fully instrumented photobioreactor that will serve to cultivate Cyanobacteria under well defined and controlled conditions. This capability…

Dynamic Changes in Molecular Interactions along the Circadian Rhythm (Orr-LDRD Membrane Biology GC, PNNL Scope #90001)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Grand Challenge

One of the most challenging tasks in cell biology is the study of the molecular interactions in the natural cellular environment in order to gain information about their dynamic changes in time and…

Data Assimilation, Visualization, and Mining

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Elena Peterson

Project type

Grand Challenge

Systems biology research involves the generation of large volumes of data using a number of different technologies such as proteomics, transcriptomics, matabolomics, and high resolution imaging…

Cyanobacteria Membrane Biology Grand Challenge: Systems Analysis of the Dynamics of Membrane Architecture, Composition, and Function- Proteomic, Metabolomic, and Metallomic Characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Project type

Grand Challenge

The strain Cyanothece sp. ATCC 51142, isolated from benthic waters off the coast near Galveston, Texas, is a unicellular strain approximately 4-7 &#956;m in diameter along the major axis. This…

AFM Imaging of Photo System Protein in Photosynthetic Membranes (Lu's LDRD-EMSL GC Biomembranes, PNNL Scope #90001)

Lead Institution

Bowling Green State University

Principal Investigator

H. Peter Lu

Project type

Grand Challenge

AFM imaging will be used to characterize photo system protein distributions and aggregations in photosyntetic membranes.

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

Manganese is an essential trace element that all organisms require. Often, this metal is found in the enzyme?s active site, such as in ribonucleotide reductase, manganese-catalase, and manganese…

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

As complete genome sequence information became available for many organisms, a novel family of proteins containing a tandem pentapeptide repeat was discovered that can be approximately described by…

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Project type

Grand Challenge

As a part of Grand Challenges in Membrane biology, Synechocystis sp. will be investigated in regards of cell morphology and elemental analysis of minerals associated with their outer and inner…

Thrust area 4: How redox proteins on the exterior of the outer membrane of Shewanella accomplish interfacial electron transfer to the Fe(III) oxide surface.

Lead Institution

University of Wyoming

Principal Investigator

Carrick Eggleston

Project type

Grand Challenge

A multidisciplinary team of scientists is investigating the mechanisms of electron transfer from metal-reducing bacteria to Fe(III) oxide surfaces as part of a PNNL/DOE-BER Grand Challenge in…

Thrust area 1: The surface structure of Shewanella and the localization of outer membrane-associated proteins hypothesized to be involved in electron transfer from metal-reducing bacteria to metal oxide surfaces.

Lead Institution

University of Guelph

Principal Investigator

Terry Beveridge

Project type

Grand Challenge

A multidisciplinary team of scientists is investigating the mechanisms of electron transfer from metal-reducing bacterial to Fe oxide surface as part of a PNNL/DOE-BER Grand Challenge in…

Thrust Area 3:Mutagenesis and Functional Characterization of Shewanella oneidensis Genes Involved in Fe(III) and Mn(IV) Oxide Reduction (LDRD #3)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alex Beliaev

Project type

Grand Challenge

Although microbial metal reduction has been extensively studied from standpoints of ecology, physiology, genetics and biochemistry, little is known about the molecular mechanisms and pathways of…

Thrust Area 1: Purification and Biophysical Characterization of MR-1 Redox Proteins (LDRD 1: Specific Aims 5&6)

Lead Institution

University of Alabama

Principal Investigator

Michael Bowman

Project type

Grand Challenge

A facultative anaerobic bacterium, Shewanella oneidensis MR-1 is able to use metal oxides, such as Mn(IV) and Fe(III), as terminal electron acceptors during anaerobic respiration. Since Mn(IV) and…

Thrust area 1: metal-reducing Shewanella believed to be involved in the dissimilatory reduction of solid phase iron (III).

Lead Institution

University of East Anglia

Principal Investigator

David Richardson

Project type

Grand Challenge

Statement of Work to be performed at the University of East Anglia, Norwich, UK A multidisciplinary team of scientists is investigating the mechanisms of electron transfer from metal-reducing…