David Hoyt

Spatiotemporal mapping of lignocellulose decomposition by a naturally evolved fungal garden microbial consortium

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kristin Burnum-Johnson

Some microbial communities composed of bacteria and fungi can readily breakdown plant matter into its component sugars. Leaf-cutter ants take advantage of such microbial communities by maintaining…

Expanding metabolomics sample preparation with new desalting protocols for enhanced molecular coverage

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Metabolomics workflows are a cornerstone of EMSL’s user program. Routinely, samples are received which are rich in inorganic components such as salts, trace metals, and minerals. These ‘salts’ impede…

Analysis of bio-oils using nuclear magnetic resonance and electron paramagnetic resonance

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Asanga Padmaperuma

NMR and EPR will be used to identity and quantify (if possible) the different functional groups and free radical species present in various bio-oils. We intent to define chemical transformations,…

Deciphering Microbial Communication Through Metabolites

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

Technology Maturation: Further development and demonstration of a high-pressure magic angle spinning (MAS) probe for use in nuclear magnetic resonance (NMR)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

As a result of a multiyear LDRD effort on the Carbon Sequestration Initiative, a new MAS capability was developed for NMR analysis of materials that would potentially be used in underground CO2…

EVALUATION OF CEMENT COMPOSITES, ROCKS, AND OTHER MATERIALS CHEMISTRY, PERMEABILITY, AND MORPHOLOGY

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Metabolomics of Mixed Microbial Communities: Understanding the Influence of Gut Microbiota on Host Energy Regulation.

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

Understanding the complex relationships in mixed microbial communities and host-microbiome interactions directly addresses the EMSL Science Theme of Biological Interactions and Dynamics: “Developing…

Development of high-throughput metabolomics technologies: Application to studying the flowering time in Arabidopsis thaliana

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

The future trajectory of metabolomics and its applications largely depends on the analytical capabilities of NMR and MS and their combination. Although the use of both NMR and MS methods is…

Fracking Optimization and In-Situ Characterization of Rock and Other Materials Permeability, Chemistry and Fracture Distributions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

Fracking Optimization and In-Situ Characterization of Rock Permeability and Fracture Distributions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Enhanced Geothermal Systems (EGS) are the future of geothermal energy, and geothermal is the future of energy production within the US and worldwide. However, to our knowledge, no prior EGS project…

Wet Non-Aqueous Supercritical Fluid Interactions with Densified Model Rock Materials

Lead Institution

St. Mary's College of Maryland

Principal Investigator

Geoffrey Bowers

Project type

Large-Scale EMSL Research

Our research program investigates the fundamental molecular-scale dynamics, energetics, and reactivity of geochemically relevant solid-fluid interfaces by integrating experimental spectroscopy and…

Integrated genomic/transcriptomic/secretomic study of plant-fungal interactions between pines and their symbiotic ectomycorrhizal fungi in the mushroom genus Suillus

Lead Institution

Duke University

Principal Investigator

Rytas Vilgalys

Project type

FICUS Research

We propose an integrated -omic study of symbiotic interactions between pines (Pinus spp.) and their ectomycorrhizal fungi (EMF) in the genus Suillus. We are developing the Pine-Suillus symbiosis as a…

Deciphering Microbial Communication Through Metabolites

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

In Situ Nuclear Magnetic Resonance Investigations of Trapping Mechanisms in CO2 Storage

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

Global increases in carbon emissions and the rapid decrease in energy securities have resulted in an increased global commitment to develop technology for stabilizing CO2 released into the…

Microbial controls on biogeochemical cycling in deep subsurface shale carbon reservoirs

Lead Institution

Colorado State University

Principal Investigator

Kelly Wrighton

Project type

FICUS Research

Terrestrial deep shales and their interfaces are carbon-rich environments that represent a significant component of the U.S. energy portfolio. Energy resources in these environments have recently…

Can Microbial Ecology and Mycorrhizal Functioning Inform Climate Change Models?

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

EMSL Staff Proposal: Using archived samples from forest Free Air CO2 Enrichment (FACE) experiments, field measurements from the Marcell Experimental Forest (MEF) and the Spruce and Peatland Response…

Experimental Capabilities and Fundamental Science for Geologic Carbon Sequestration: The PNNL In Situ Supercritical Suite (IS3)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kevin Rosso

Project type

Large-Scale EMSL Research

This Science Theme proposal requests EMSL access for investigators in the In Situ Supercritical Suite (IS3) component of PNNL's Carbon Sequestration Initiative (CSI). The CSI is a multi-million…

Development of an Integrated EMSL MS and NMR Metabolic Flux Analysis Capability In Support of Systems Biology: Test Application for Biofuels Production

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Isern

An important element in the production of biofuels is the metabolic engineering process required to maximize the production of renewable biofuel molecules from fermentable substrates. Biofuel…

EVALUATION OF CEMENT COMPOSITES, ROCKS, AND OTHER MATERIALS CHEMISTRY, PERMEABILITY, AND MORPHOLOGY

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Catalysis Research Campaign: An Integrated Multi-Capability Approach for Analysis of Catalytic Systems

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Charles Peden

Project type

Research Campaign

In this research campaign, advanced capabilities within the Environmental Molecular Sciences Laboratory (EMSL) at the Pacific Northwest National Laboratory will be integrated for in-depth, molecular…

Metabolomics of Mixed Microbial Communities: Understanding the Influence of Gut Microbiota

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

Understanding the complex relationships in mixed microbial communities and host-microbiome interactions directly addresses the EMSL Science Theme of Biological Interactions and Dynamics: “Developing…

Elucidating synergistic plant-bacteria metabolic pathways for alleviating iron deficiency

Lead Institution

Oregon State University

Principal Investigator

Rene Boiteau

Nutrient mobilization from soil minerals is critical for plant growth, particularly in marginal lands with high pH soils or low phosphate availability. One way that rhizospheric bacteria enhance…

Optical and structural properties of doped ZnO nanocrystals

Lead Institution

Washington State University

Principal Investigator

Matthew Mccluskey

Project type

Exploratory Research

Zinc oxide (ZnO) is a semiconductor that has attracted resurgent interest as an electronic material for energy applications. With a direct gap of 3.4 eV at room temperature, ZnO is a wide band gap…

Developing Signatures that Relate Fecal Microbiome Characteristics with Patient Phenotype

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Isern

The exploration of the human microbiome is currently an area of keen interest to NIH and biotech firms, and has been explored by many researchers using either NMR or MS metabolomics or 16S rDNA…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

Role of Human Intestinal Microbiota on Success of Surgical Weight Loss Procedures: Enhanced Understanding through application of NMR-based Metabolomics - Preliminary results

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

We plan to use high-resolution 1H NMR spectroscopy to compare metabolite spectral profiles in aqueous extracts of human fecal material to obtain metabolic profiles significant to predicting outcomes…

Fracturing Optimization and In-Situ Characterization of Rock Permeability and Fracture Distributions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Enhanced Geothermal Systems (EGS) are the future of geothermal energy, and geothermal is the future of energy production within the US and worldwide. However, to our knowledge, no prior EGS project…

EVALUATION OF CEMENT COMPOSITES, ROCKS, AND OTHER MATERIALS CHEMISTRY, PERMEABILITY, AND MORPHOLOGY

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Experimental Capabilities and Fundamental Science for Geologic Carbon Sequestration: The PNNL In Situ Supercritical Suite (IS3)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Loring

Project type

Large-Scale EMSL Research

This Science Theme proposal requests EMSL access for investigators in the In Situ Supercritical Suite (IS3) component of PNNL's Carbon Sequestration Initiative (CSI). The CSI is a multi-million…

Biogeochemical and genomic determinants of methane cycling in freshwater wetland sediments

Lead Institution

Colorado State University

Principal Investigator

Kelly Wrighton

Project type

Large-Scale EMSL Research

Terrestrial freshwater wetlands are carbon rich environments that play a significant role in the global release of methane to the atmosphere. Our research provides an understanding of how methane…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

Rhizosphere Research Campaign: The plant-soil interface - understanding dynamic interactions in the context of environmental change

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

Project type

Research Campaign

The ultimate, long-term goal is to determine what drives source-sink relationships that control the cycling of plant nutrients belowground. To fully appreciate the complexities of this dynamic…

Developing Signatures that Relate Fecal Microbiome Characteristics with Patient Phenotype

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

The exploration of the human microbiome is currently an area of keen interest to NIH and biotech firms, and has been explored by many researchers using either NMR or MS metabolomics or 16S rDNA…

EVALUATION OF CEMENT COMPOSITES, ROCKS, AND OTHER MATERIALS CHEMISTRY, PERMEABILITY, AND MORPHOLOGY

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Dynamical Behavior of Hydrocarbons Under Nanopore Confinement

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karl Mueller

It is becoming increasingly clear that organic molecules present as gas species, in aqueous and mixed-volatile fluids - ranging from simple hydrocarbons and carboxylic acids to branched and cyclic…

Metabolomics of Mixed Microbial Communities: Understanding the Influence of Gut Microbiota

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

Combining NMR data and Mass Spec data with biostatistics and bioinformatics will enhance the impact of phylogenetic analysis, biochemical mechanistic studies, and mathematical modeling currently…

A User Interface Framework for Rigorous and Automated NMR Metabolomics Workflows

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Robert Young

NMR is powerful tool to study complex systems due to its non-destructive, quantitative, and global profiling capability making it an indispensable part of metabolomics research. EMSL's NMR…

Metabolomics of Mixed Microbial Communities: Understanding the Influence of Gut Microbiota on Host Energy Regulation.

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

Understanding the complex relationships in mixed microbial communities and host-microbiome interactions directly addresses the EMSL Science Theme of Biological Interactions and Dynamics: “Developing…

Deciphering controls on plant decomposition in Arctic ecosystems: Identifying unknown microbial condensed tannin degradation pathways

Lead Institution

Colorado State University

Principal Investigator

Kelly Wrighton

Project type

FICUS Research

Climate change in the Arctic is progressing rapidly, and much of the focus, thus far, has been on permafrost thaw and the rapid mineralization of soil organic carbon (C) by soil microorganisms…

EVALUATION OF CEMENT COMPOSITES, ROCKS, AND OTHER MATERIALS CHEMISTRY, PERMEABILITY, AND MORPHOLOGY

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Further development and demonstration of a high-pressure magic angle spinning (MAS) probe for use in nuclear magnetic resonance (NMR)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

Through a multiyear LDRD effort on the Carbon Sequestration Initiative, a new MAS probe was developed for NMR analysis of materials that would potentially be used in underground CO2 sequestration…

Tracking the Fate of new C in Northern Peatlands by a Compound-Specific Stable Isotope-Labeling Approach coupled with FISH-NanoSIMS

Lead Institution

University of Arizona

Principal Investigator

Malak Tfaily

Here, we propose to examine the priming effect, drivers, and dynamics of the "new C" that is stimulated by climate change in Northern peatlands soil using a stable isotope labeling approach…

Evaluation of cement composites, rocks, and other materials chemistry, permeability, and morphology

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Carlos Fernandez

Through this proposal EMSL will provide experimental resources for enhanced energy production and subsurface science discovery that supports the needs of DOE and the nation. These proposed…

Projects