Tracking the Fate of new C in Northern Peatlands by a Compound-Specific Stable Isotope-Labeling Approach coupled with FISH-NanoSIMS
EMSL Project ID
49551
Abstract
Here, we propose to examine the priming effect, drivers, and dynamics of the "new C" that is stimulated by climate change in Northern peatlands soil using a stable isotope labeling approach in a long term incubation experiment. The aim is to understand: 1) how changes in labile carbon inputs drive microbial community composition, 2) how microbial communities contribute to CH4 and CO2 fluxes, and 3) the impact of the labile new C on ecosystem C storage or release. We will trace the fate of 13C-glucose added to decomposition incubations into (1) different organic matter (through high resolution 21T FT-ICR-MS and GC-FTMS), then into (2) microbial community (16S rRNA), and finally into (3) greenhouse gases (CO2 and CH4 gas emissions). These techniques will be complemented by FISH-NanoSIMS to detect, visualize, and validate the complex metabolic behavior observed within these soil communities. Finally, we will leverage PNNL investments in the Signature Discovery Initiative (SDI) to implement informatics tools that allow us to integrate these capabilities together to better understand ecosystem processes. The proposed research represents the first fully integrated study to achieve mechanistic understanding of substrate transformation through the microbial loop, and will help shed new light on the mechanistic basis of biological processes and how these processes change in response to community interactions and shifting environmental conditions. In particular, this study will provide insights into the in situ function of microorganisms and improve EMSL capabilities for understanding of the impacts of climate change on soil-plant-microbial interactions that control C cycling in Northern peatlands.
Project Details
Start Date
2017-02-23
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
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Lynch L.M., M. Machmuller, C.M. Boot, T.P. Covino, C.D. Rithner, F. Cotrufo, and D.W. Hoyt, et al. 2019. "Dissolved organic matter chemistry and transport along an Arctic Tundra Hillslope." Global Biogeochemical Cycles 33, no. 1:47-62. PNNL-SA-136691. doi:10.1029/2018GB006030
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