Structural Genomics Collaborative Access Team (CAT)
EMSL Project ID
11701
Abstract
The Structural Genomics CAT will carry out research focused in structural genomics and related research areas with a primary goal of increasing throughput, maximizing efficiency of existing resources, and developing technology to increase throughput and efficiency.A significant amount of CAT's resources will support the research objectives of the NorthEast Structural Genomics consortium (NESG), a consortium of 10 institutions involving 23 NMR scientists. The NESG objective is to produce a high volume of protein structure data and to test the feasibility of high throughput, NMR-based Structural Genomics, as per the guidelines of the National Institutes of Health (NIH) Protein Structure Initiative (PSI). Structural Genomics defines an ultimate goal of determining the three-dimensional structure of all proteins encoded by an organism's genome. Each three-dimensional structure can either be derived experimentally, or given a sufficient sequence similarity, can be modeled by homology from an appropriate experimentally derived structure. An important objective of the NIH PSI pilot centers is to test feasibility of various approaches for high throughput Structural Genomics. A critical concept being assessed is what role that NMR spectroscopy should play in Structural Genomics.
Other Structural Genomics CAT projects include a Lawrence Berkeley National Laboratory (LBNL) structural biology project led by Steve Holbrook, focused on solving the structure of whole classes of DNA repair proteins, a Battelle/Chiron project involving Dirk Bussierre looking at essential genes in S. aureus, and a Battelle supported project involving Fred Ausubel at Massachusetts General Hospital, Harvard Medical School, looking at the structure of large groups of proteins encoded by essential genes in Pseudomonas aeruginosa.
The CAT will partition its time among several activities. A major objective is high throughput protein structure determination. In support of this objective, a considerable fraction of the resources will be used for data collection for NESG and the other NIH supported PSI pilot centers. Another objective is development of new pulse sequences and approaches for reducing the overall resource required for each protein structure determination. A final objective of the CAT is to develop automation technologies for data collection and data management.
Project Details
Start Date
2004-11-23
End Date
2006-09-26
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Everett JK, R Tejero, SB Murthy, T Acton, JM Aramini, M Baran, J Benach, JR Cort, A Eletsky, F Forouhar, R Guan, AP Kuzin, HW Lee, G Liu, R Mani, B Mao, JL Mills, AF Montelione, K Pederson, R Powers, TA Ramelot, PM Rossi, J Seetharaman, D Snyder, GVT Swapna, SM Vorobiev, Y Wu, R Xiao, Y Yang, CH Arrowsmith, JF Hunt, MA Kennedy, JH Prestegard, T Szyperski, L Tong, and G Montelione. 2016. "A Community Resource of Experimental Data for NMR / X-ray Crystal Structure Pairs." Protein Science 25(1):30-45. doi:10.1002/pro.2774
Yin C, JM Aramini, LC Ma, JR Cort, GVT Swapna, RM Krug, and G Montelione. 2011. "Backbone and Ile-?1, Leu, Val Methyl 1H, 13C and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein." Biomolecular NMR Assignments 5(2):215-219. doi:10.1007/s12104-011-9303-8