Mass Spectrometry Imaging of Microbial Communities
EMSL Project ID
44679
Abstract
Despite our significant technological advancements, the solutions to many of humanities lingering problems such as bioremediation of toxic heavy metal environmental contaminants, creation of renewable and biodegradable energy solutions, biofouling, and discovery of novel therapeutics for battling antibiotic resistant microbial pathogens are likely to be found through understanding and exploiting the natural behavior of microbial communities. Due to the complexity of biological systems, no single technique can provide chemical characterization with a sufficient level of detail needed to form accurate hypotheses. As compounds involved in microbial extracellular signaling networks are widely varied and include elemental nutrients (e.g. Na+, K+, Mg+, and Fe+), small metabolites, lipids, peptides and proteins, we will combine newly developed MS imaging approaches with optical and electron microscopy and state-of-the-art 'omics' approaches (proteomics, metabolomics) to comprehensively characterize the chemical composition of the extracellular material. Through the use of EMSL's extensive mass spectrometry and spectroscopy facilities, we will be able to utilize a multimodal analytical approach to study these complex signaling networks using established MALDI-TOF and TOF-SIMS IMS methodologies in combination with novel IMS approaches including nanoDESI-LTQ/FT and C60 SIMS on an FT-ICR instrument. Each of these instruments displays a particular molecular bias that when combined can paint a more complete picture of the molecular make-up of a particular biological system. Furthermore, MS imaging results will be compared to the results of fluorescence, optical, and electron microscopy experiments to determine how molecular changes in the composition of the extracellular material translate into structural alterations. By implementing this research strategy to study bioremediation, such as the reduction of heavy metal oxides by Shewanella oneidensis MR1, and quorum sensing and biofilm formation by commensal and pathogenic bacteria, such as Staphylococcus aureus and Pseudomonas aeruginosa, we will be able to make significant strides in the duplications and exploitation of these processes for the betterment of the environment and human health.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Lanekoff I, J Cha, JE Kyle, SK Dey, J Laskin, and KE Burnum-Johnson. 2016. "Trp53 deficient mice predisposed to preterm birth display region-specific lipid alterations at the embryo implantation site." Scientific Reports. doi:10. 1038/srep33023
Lanekoff IT, BS Heath, AV Liyu, M Thomas, JP Carson, and J Laskin. 2012. "An Automated Platform for High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 84(19):8351-8356.
Lanekoff IT, KE Burnum-Johnson, M Thomas, JTL Short, JP Carson, J Cha, SK Dey, P Yang, MC Prieto Conaway, and J Laskin. 2013. "High-Speed Tandem Mass Spectrometric in Situ Imaging by Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 85(20):9596-9603. doi:10.1021/ac401760s
Lanekoff IT, M Thomas, and J Laskin. 2014. "Shotgun Approach for Quantitative Imaging of Phospholipids Using Nanospray Desorption Electrospray Ionization Mass Spectrometry ." Analytical Chemistry 86(3):1872-1880. doi:10.1021/ac403931r
Lanekoff IT, M Thomas, JP Carson, JN Smith, C Timchalk, and J Laskin. 2013. "Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 85(2):882-889. doi:10.1021/ac302308p
Lanekoff IT, OV Geydebrekht, GE Pinchuk, A Konopka, and J Laskin. 2013. "Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization." Analyst 138(7):1971-1978. doi:10.1039/C3AN36716A
Mohimani H, R Kersten, W Liu, M Wang, SO Purvine, S Wu, HM Brewer, L Pasa-Tolic, N Bandeira, BS Moore, PA Pevzner, and PC Dorrestein. 2014. "Automated genome mining of ribosomal peptide natural products." ACS Chemical Biology 9(7):1545-1551. doi:10.1021/cb500199h
Watrous JD, PJ Roach, BS Heath, T Alexandrov, J Laskin, and PC Dorrestein. 2013. "Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry." Analytical Chemistry 85(21):10385-10391. doi:10.1021/ac4023154
Watrous JD, PJ Roach, T Alexandrov, BS Heath, JY Yang, R Kersten, M vander Voort, K Pogliano, H Gross, JM Raaijmakers, BS Moore, J Laskin, N Bandeira, and PC Dorrestein. 2012. "Mass Spectral Molecular Networking of Living Microbial Colonies." Proceedings of the National Academy of Sciences of the United States of America 109(26):E1743-E1752. doi:10.1073/pnas.1203689109