Skip to main content

Discovery of Host Proteins Involved in Host-Pathogen Interactions


EMSL Project ID
47623

Abstract

The multiple mechanisms by which pathogenic enterobacteria of the genus Salmonella infect their host indicate a complex host-pathogen relationship. The pathogen secretes dozens of proteins, termed 'effectors', in an organized strategy to modulate host cell function and evade the host immune response to enable colonization of the host. Recently, we discovered more than 50 new secreted proteins presumably deployed by Salmonella to subvert host cell defense mechanisms, bringing the total number of identified putative secreted effectors to nearly 100. Identifying the host proteins targeted by these putative effectors and determining the nature of these interactions will enable development and testing of mechanistic hypotheses about their modes of action.
Our research plan employs a novel high throughput proteomic screening approach to identify effector and host proteins involved in specific host-pathogen interactions and to elucidate the underlying molecular mechanisms behind these interactions. As part of this plan, we will collaborate with the Protein Structure Initiative 3 (PSI:Biology) network to generate essential structural information to help define the mechanisms by which the pathogen modulates or disrupts host cell functions. The long-term goal is to identify novel therapeutic targets and develop new tools for manipulating the host cell response to infection.

Project Details

Start Date
2012-09-07
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Joshua Adkins
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

Charles Ansong
Institution
National Institutes of Health

Team Members

Geremy CD Clair
Institution
Pacific Northwest National Laboratory

Christina Stevenson
Institution
Pacific Northwest National Laboratory

Eric Merkley
Institution
Pacific Northwest National Laboratory

Ernesto Nakayasu
Institution
Pacific Northwest National Laboratory

Roslyn Brown
Institution
Washington State University Tri-Cities

Heather Olson
Institution
Environmental Molecular Sciences Laboratory

Fred Heffron
Institution
Oregon Health & Science University

John Cort
Institution
Pacific Northwest National Laboratory