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Substrate Oxidation and Protein Synthesis Integration in a Complex Biological System


EMSL Project ID
47729

Abstract

These investigations will explore the complex interactions involved in cellular metabolism and protein synthesis in accordance with the EMSL Science Theme: Biological Interactions and Dynamics. We will use a complex model employing the pig neonatal heart and brain in vivo to link intra-cellular regulatory networks that control the response of cells to their environment. The neonatal heart in particular provides an excellent model for all cell systems as it undergoes rapid growth through cell proliferation and expansion and adapts to quickly to physiological changes. The neonatal brain is also undergoing rapid development but is extremely vulnerable to insults. These studies will have broad applications to protein synthesis and metabolomics extending past our models. Regulation of these pathways is relatively conserved through evolution. Therefore, the defined principles and techniques will be applicable to metabolomics and protein synthesis in microbes with specific EMSL interest.

Project Details

Start Date
2012-12-03
End Date
2013-09-30
Status
Closed

Team

Principal Investigator

Michael Portman
Institution
Seattle Children's Research Institute

Team Members

Aaron Olson
Institution
Seattle Children's Research Institute

Nancy Isern
Institution
Environmental Molecular Sciences Laboratory

Related Publications

Files MD, M Kajimoto, CM Priddy, DR Ledee, C Xu, C Des Rosiers, NG Isern, and MA Portman. 2014. "Triiodothyronine facilitates weaning from extracorporeal membrane oxygenation (ECMO) by improved mitochondrial substrate utilization." Journal of the American Heart Association 3(2):Article No. e000680. doi:10.1161/jaha.113.000680
Kajimoto M, CM Priddy, D Ledee, C Xu, NG Isern, A Olson, C Des Rosiers, and MA Portman. 2013. "Myocardial Reloading after Extracorporeal Membrane Oxygenation Alters Substrate Metabolism While Promoting Protein Synthesis." Journal of the American Heart Association 2(4):Artcicle No. e000106. doi:10.1161/JAHA.113.000106
Kajimoto M, DR Ledee, NG Isern, and MA Portman. 2017. "Right ventricular metabolism during venoarterial extracorporeal membrane oxygenation in immature swine heart in vivo." American Journal of Physiology. Heart and Circulatory Physiology 312(4):H721-H727. doi:10.1152/ajpheart.00835.2016