Projects

Vertical profile of atmospheric particle composition via tethered balloon system

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Fan Mei

Project type

Exploratory Research

The vertical distribution of aerosol strongly influences the radiative forcing of the aerosols. However, observational data is limited. Both satellite observation and ground-based measurements are…

The effects of coculture on cellular spatial heterogeneity in colony and plant-associated biofilms

Lead Institution

University of Massachusetts Medical School

Principal Investigator

Elizabeth Shank

Project type

Exploratory Research

Bacteria live everywhere, from the soil and sea to the surface of every plant and animal on earth. Most bacteria exist within diverse, multispecies communities that exert profound impacts on their…

Character of organic matter in soils of temperate forest ecosystems: Do chlorophyll catabolites (phyllobilins) travel deep?

Lead Institution

Cornell University

Principal Investigator

Carmen Martinez

Project type

Exploratory Research

Soil organic matter (SOM) is the largest reservoir of carbon (C) and nutrients, especially nitrogen (N), in terrestrial ecosystems. SOM is found throughout soil profiles, with content typically…

Unravelling The Mechanisms Of Biomineralization In A Biogeochemically Significant Microbe Through Multimodal Nano-Imaging At The Organic-Inorganic Interface

Lead Institution

University of Washington

Principal Investigator

Alexander Gagnon

Project type

Exploratory Research

The pace of skeletal growth in shells and bones during biomineralization is impacted by interactions between biologically produced organic compounds and the growing mineral. The organic component…

Mineralogy and Soil Organic Matter Composition as Drivers of Reactive Nitrogen Emissions from Midwestern Hardwood Forest Soils

Lead Institution

Indiana University - Bloomington

Principal Investigator

Jonathan Raff

Project type

Exploratory Research

The overall goal of this proposal is to investigate how plant-microbe-mineral interactions drive emissions of reactive nitrogen from soils. Fluxes of reactive nitrogen oxides [NOy = NO + NO2 + HONO]…

Quantifying Peptide Abundance Ratios

Lead Institution

North Carolina State University

Principal Investigator

Michael Goshe

Project type

Exploratory Research

Regulation of protein expression and modulation of post-translational modifications (PTMs) are biochemical processes that are vital to cellular function. Phosphorylation and glycosylation are two of…

3D Flow and transport in highly heterogeneous porous media

Lead Institution

Oregon State University

Principal Investigator

Brian Wood

Project type

Exploratory Research

We will be examining flow and transport, specifically macrodispersion, in highly heterogeneous media in 3-dimensions. Particular focus will be on tailing phenomena that lead to non-Fickian spreading…

Multiple Sensor Data Integration (PNNL Scope # 90001)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nancy Valentine

Project type

Exploratory Research

Development of a mathematical/statistical framework for nuclear, chemical, and biological (NCB) sensor integration and decision analysis is proposed. In particular, a statistical model for…

Towards a systems model of circadian control of gene function to regulate metabolic phenotype and cellulase production in Neurospora crassa

Lead Institution

Rensselaer Polytechnic Institute

Principal Investigator

Jennifer Hurley

Project type

Large-Scale EMSL Research

Biofuel production using cellulases extracted from fungi has the potential to be a major resource to replace fossil fuels, and regulating the synthesis of these and other enzymes is crucial for the…

Exploring the native cell wall nanoarchitecture of engineered bioenergy crops via multi-dimensional solid-state NMR.

Lead Institution

University of Adelaide

Principal Investigator

Jennifer Mortimer

Project type

Large-Scale EMSL Research

Carbon dioxide from the atmosphere, an inorganic carbon source, is fixed by plants via photosynthesis to form the polysaccharides and lignin that constitute the plant cell wall. The plant cell wall,…

Selective Sensors for High Spatiotemporal Imaging of Small Molecule Targets in Metabolic Pathways of Plants and Cells involved in Bio-energy Processes

Lead Institution

University of Texas at Austin

Principal Investigator

Yi Lu

Project type

Large-Scale EMSL Research

As a member of the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), a DOE Bioenergy Center at the University of Illinois at Urbana-Champaign, we aim to develop selective sensors to…

Cryo-FIB and High-Vacuum Cryo-Transfer for Atom Probe Tomography Analysis of Organic Molecular Materials

Lead Institution

Colorado School of Mines

Principal Investigator

Jeramy Zimmerman

Project type

Large-Scale EMSL Research

The morphology of mixed molecular solids impacts macroscopic properties of the material (e.g. conductivity). Our group has developed atom probe tomography (APT) analysis methods for organic small…

Identifying dynamic sources and transformations of dissolved organic matter in coastal watersheds exposed to extreme flooding

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nicholas Ward

Project type

Large-Scale EMSL Research

The primary objective of this proposal is to improve mechanistic understanding of the hydrobiogeochemical processes governing the cycling of organic matter (OM) across the land-to-sea continuum…

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

Rhodobacter sphaeroides is an alpha-proteobacterium which can remodel its cell envelope under low-oxygen or anaerobic conditions, by increasing its lipid content and forming intracytoplasmic membrane…

The Diurnal Redox Proteome of Chlamydomonas

Lead Institution

Michigan State University

Principal Investigator

Daniela Strenkert

Project type

Large-Scale EMSL Research

Nearly all organisms exhibit diurnal metabolic cycles, especially phototrophs, whose metabolism is strictly dependent on light. Transcriptomics experiments in cyanobacteria, algae and plants have…

Characterizing role of particle-water in processing of biomass burning emissions and SOA formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

ManishKumar Shrivastava

Project type

Large-Scale EMSL Research

Secondary organic aerosol (SOA) formed by multiphase chemical reactions in the atmosphere greatly contribute to fine particulate matter and organic aerosols (OA). Despite mounting evidence, aqueous-…

Chemical Imaging and Ice Nucleation Properties of Individual Arctic Atmospheric Particles

Lead Institution

University of Michigan

Principal Investigator

Kerri Pratt

Project type

Large-Scale EMSL Research

The proposed project will address critical gaps in our understanding of the Arctic aerosol sources, chemical composition, and ice nucleation properties through chemical imaging and ice nucleation…

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

Large-Scale EMSL Research

Determining in situ metabolic processes and interactions among diverse assemblages of microorganisms remains a critical challenge in microbial ecology. Consequently, identifying novel genes and…

Environmental Photochemical Catalysis and Microphysical Properties of Fe-Organic Aerosols

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Project type

Large-Scale EMSL Research

The intent of this collaborative research is to investigate photo-catalytic effects of atmospheric aging on the chemical composition and the microphysical properties of secondary organic aerosol (SOA…

Connecting Structure and Function in Anaerobic Fungal Cellulosomes

Lead Institution

University of California, Santa Barbara

Principal Investigator

Michelle O'Malley

Project type

Large-Scale EMSL Research

This proposal seeks use of EMSL resources to characterize the unique, multi-enzyme cellulosomes made by anaerobic gut fungi that are found within large herbivores. Cellulosomes enable anaerobic…