Projects

As a scientific user facility, the Environmental Molecular Sciences Laboratory (EMSL) provides expertise, instrumentation, and resources to 250 projects each year.

EMSL projects displayed on the EMSL web site project page reflect project abstracts and information migrated to this page before April 30, 2024. To review current and past EMSL user projects, visit EMSL Science Central.™

Probing the Extreme Temperature and Salinity Limits of Deep Subsurface CH4-cycling Chemolithoautotrophic Microbial Syntrophic Communities and Their Assembly Using NanoSIMS and FISH-TAMB

Lead Institution

Princeton University

Principal Investigator

Tullis Onstott

Project type

Large-Scale EMSL Research

The specific aim of this Large-Scale EMSL proposal is to image the metabolic interactions of deep subsurface anaerobic CH4-cycling microbial consortia: 1) at high pressure and temperature; 2) at high…

Nature of Natural Organic Matter Interactions with Mineral Surfaces

Lead Institution

St. Mary's College of Maryland

Principal Investigator

Geoffrey Bowers

Project type

Large-Scale EMSL Research

The interactions of organic- and bio-molecules with minerals play a central role in controlling soil biogeochemistry; plant nutrition; chemical processes in sediments, soils, sedimentary rocks and…

Determining Structures of the APOBEC3-HIV-1 Vif-E3 Ligase Complexes

Lead Institution

University of Minnesota

Principal Investigator

Nadine Shaban

The APOBEC3 family of cytosine deaminases are antiviral factors that can restrict the replication of HIV-1. The virus eliminates this threat by encoding a small protein called Vif that hijacks the…

Visualizing Uranium Atom Exchange in UO2 Driven by Biogeochemical Processes

Lead Institution

Washington University in St. Louis

Principal Investigator

Daniel Giammar

Project type

Large-Scale EMSL Research

Redox-driven recrystallization of metal oxides is an exciting environmental phenomenon that can influence biogeochemical cycles of both nutrients and contaminants. This phenomena occurs in systems…

Functional characterization of a defined Panicum rhizobiome

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Esther Singer

Project type

Large-Scale EMSL Research

Nitrogen limitation is a global challenge for biofuel crop resilience and hence bioenergy security. Plant growth promoting bacteria (PGPB) can help to mitigate plant stress by forming symbiotic…

Manganese biomineralization by the bacterial protein complex Mnx

Lead Institution

University of Washington

Principal Investigator

Bradley Tebo

Project type

Large-Scale EMSL Research

Manganese-oxide mineral formation by bacterial oxidation of dissolved Mn(II) is an important process in aquatic and soil environments and is a key part of the global Mn cycle. The Mn bio-oxides…

Mechanisms of Nitrogen Fixation by Endophytes of Populus

Lead Institution

University of Washington

Principal Investigator

Sharon Doty

Project type

Large-Scale EMSL Research

Native poplar (Populus) plants have a diverse microbiota including endophytes, microorganisms that live within plants, some of which can fix atmospheric nitrogen (N2) into forms of nitrogen (N)…

Probing the Martian atmosphere and hydrosphere using micro and nanoscale analyses

Lead Institution

Royal Ontario Museum

Principal Investigator

Kimberly Tait-Sena

Project type

Large-Scale EMSL Research

The aim of this study is to fully characterize the micro- and nano-structural shock effects in Martian phosphate and sulfide minerals using cutting-edge EMSL instrumentation including: Transmission…

Studying the Evolution of Atmospheric Aerosol at the High-altitude Mountain Station of Mt. Cimone, Italy

Lead Institution

CNR - Consiglio Nazionale delle Ricerche

Principal Investigator

Angela Marinoni

Project type

Large-Scale EMSL Research

Free tropospheric air contains a mixture of aerosol from far sources -- through long-range transport -- as well as, local emissions -- through vertical mixing of air from the planetary boundary layer…

Community metabolism and greenhouse gas fluxes in industrial salt ponds pre- and post-restoration

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Susannah Tringe

Project type

Large-Scale EMSL Research

Coastal environments are a source of significant uncertainty in global climate models, and have historically been subjected to large-scale manipulations with substantial impact on the global carbon…

Identifying and understanding the function and importance of vertically transferred microorganisms within a bioenergy crop microbiome

Lead Institution

Michigan State University

Principal Investigator

Lisa Tiemann

Project type

Large-Scale EMSL Research

To meet the goals of highly productive and sustainable bioenergy cropping systems, we need to explore all options for improving the efficiency of bioenergy crops, including optimizing the positive…

Measuring Physical, Chemical, and Optical Properties of Wildfire Aerosols Using Advanced Instrumentation Toward Improving Their Representation in Atmospheric Models

Lead Institution

Michigan Technological University

Principal Investigator

Lynn Mazzoleni

Project type

Large-Scale EMSL Research

We propose an integrated experimental and modeling study of biomass burning aerosol rich in spherical particles - also known as "tar balls" - using the multi-modal micro-spectroscopy,…

Managing the cellular zinc economy: proteomics-enabled predictive understanding of zinc hierarchy

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Crysten Blaby

Project type

Large-Scale EMSL Research

Zinc is an essential nutrient in biology. When encountering environments with suboptimal zinc availability, organisms initiate acclimation strategies that operate to increase assimilation and release…

Orchestrated chaos at the hyphal tip as fungi oxidatively decompose plant cell walls

Lead Institution

University of Minnesota

Principal Investigator

Jonathan Schilling

Project type

Large-Scale EMSL Research

Our research, supported by a new DOE BER Systems Biology grant (as of Sept. 2018), focuses on "brown rot" fungi that selectively extract sugars embedded in lignin. These fungi, we…

Physical and chemical properties of ice-nucleating particles in the Arctic

Lead Institution

University of British Columbia

Principal Investigator

Allan Bertram

Project type

Large-Scale EMSL Research

Ice-nucleating particles (INPs) influence the frequency and properties of clouds in the Arctic, which, in turn, influences climate. Hence, to improve predictions of climate in the Arctic, information…

Decrypting inter-cellular communication within the soil microbiome by eavesdropping on pairwise signaling between bacteria

Lead Institution

University of Pittsburgh

Principal Investigator

Erik Wright

Project type

Large-Scale EMSL Research

The soil microbiome is composed of a dense network of interacting cells that play a pivotal role in nutrient cycling and plant growth. Decrypting how soil microorganisms communicate through a wide…

Structure and Reactivity of Acetonitrile on Pt(111) and Graphene Substrates

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

R. Smith

Acetonitrile is a widely used solvent in electrochemical systems and its behavior at electrode interfaces can affect the performance of batteries and energy storage devices. In some recent work on…

Visualizing the chemical plasticity of plant roots in response to soil biotic and abiotic properties and developing a better framework to predict fine root decomposition

Lead Institution

Clemson University

Principal Investigator

Vidya Appukuttan Suseela

Project type

Large-Scale EMSL Research

Fine roots of Plants represent a major contributor to soil organic carbon storage. Despite its fundamental role in global carbon cycling, we know little about the factors controlling the residence…

Systematic characterization of heterologous protein secretion in Aspergillus niger

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

Project type

Large-Scale EMSL Research

Economical and sustainable production of biofuels and bioproducts from biomass requires efficient utilization of the carbon contained within, thereby creating a need for enzyme cocktails used in the…

Using Multi-Modal Micro-Spectroscopy Techniques to Determine the Impact of Aerosolized Organic Material on Biogeochemical Cycles of Fe and P in the Amazon Basin

Lead Institution

University of Miami

Principal Investigator

Cassandra Gaston

Project type

Large-Scale EMSL Research

Aerosols transported from Africa are thought to critically impact the biogeochemical cycling of iron (Fe) and phosphorus (P) in the Amazon and relieve nutrient limitations in this ecosystem…

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