Arunima Bhattacharjee

A model-experiment workflow for 2D pore scale micromodels from X-ray Computed Tomography (XCT) images

Lead Institution

University of Tennessee

Principal Investigator

Erin Rooney

Project type

Limited Scope

Climate change is amplifying soil disturbances with impacts to water and nutrient transport within pore networks at the aggregate scale. Pore deformation alters pore connectivity, pore throat size…

Development of Atmospheric Pressure Environmental (APE) Chamber for Chemical Imaging of Aerosol, Plant, and Soil Samples

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Zezhen Cheng

Maintaining low-pressure (∼2×10−6-10 Torr) inside the Environmental Scanning Electron Microscope (ESEM) chamber is the major challenge in pressure- and/or relative humidity (RH)-sensitive sample (e.g…

Incorporation of Real-Time Mass Spectrometry (RTMS) and Applications into EMSL’s Integrated Research Platforms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karl Weitz

We propose to install an RTMS capability on EMSL’s existing high mass resolution Agilent 7250 QTOF system for the (TAP) IRP for development of first-of-kind real-time atmospheric organic monitoring…

Investigating spatiotemporal monitoring of soil organic matter using spectral induced polarization

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Judith Robinson

Understanding soil organic matter (SOM) dynamic behavior and interactions is critical in determining regional soil fertility and health. SOM controls crop yields, the diversity of microorganisms used…

A 3D printed SubTap platform for high-throughput plant-microbe interaction studies

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Arunima Bhattacharjee

High-throughput (HTP) mass spectrometry will provide a holistic analysis of microbial and plant community interactions. The recently developed 3D printed SubTap platform (through a collaborative work…

TEAMS - Trial Ecosystems for the Advancement of Microbiome Science

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

A primary goal of the TEAMs project is to optimize the Soil EcoFAB devices to accommodate goals of the soil microbiome scientific community. We have made significant progress advancing the Soil…

Development of nanospray desorption electrospray ionization with sample imprinting as a tool to characterize microbial and below-ground interfaces

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Mass spectrometry imaging (MSI) is a well-established strategy to observe spatial molecular-level information. However, the vacuum associated with many imaging modalities do not allow for the…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

An EMSL User-ready spatially resolved metabolomics platform capable of high-lateral MS imaging and epifluorescence-based targeting

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

To upgrade our laser-ablation electrospray ionization mass spectrometry (LAESI-MS) platform to a capability that can automatically identify cells and areas of interest and acquire spatially resolved…

Soil-like habitats for spatial analysis of microbe-mineral interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Arunima Bhattacharjee

The objective of this study is to develop mineral microfluidics compatible with a suite of optical and chemical imaging capabilities at EMSL, as well as SSRL, for probing microbially relevant…

1000 Soils Research Campaign

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Project type

MONet

To more fully understand the cycling of carbon, carbon-based nutrients and key elements from the molecular to basin scale across variations in soil chemistry, the Environmental Molecular Sciences…

Deciphering exopolysaccharide functional specificity via visualization of chemical motifs that mediate microbial-microenvironment interactions in soil micromodels

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Exopolysaccharides (EPS) are carbohydrate-based matrices of microbial biofilms that microbes use to adapt to their surroundings. The chemical structure of EPS, even within single species biofilms,…

Automating Microbial Community Phenotyping within Synthetic Soil Aggregates

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jayde Aufrecht

Microbial hotspots are the sites of nearly all biogeochemical transformations (BGT) in the soil. Unfortunately, the small size (µm to mm) and transient existence (hours to days) of microbial…

Manipulation of Host Signature Expression Following Respiratory Virus Infection

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Pathogen invasion of host cells causes a myriad of functional changes including alterations of chromatin accessibility often limiting defense responses, shunting of cellular resources to centers of…

Projects