Sarah Williams

Proteome Changes Underlying Peripheral Sympathetic Dysfunction in Cardiac Disease

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Fulcher

Project Summary Myocardial infarction and heart failure increase risk for ventricular arrhythmias and sudden cardiac death. Autonomic dysregulation and sympathetic hyperactivity accompany these…

Hair Bundle Structure and Dynamics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Fulcher

A principal long-term goal of our lab is to describe the molecular steps leading to hair-bundle assembly. As the mechanosensitive organelle of a hair cell, a bundle responds to mechanical forces by…

Nanodroplet sampling processing platform for amine containing metabolites in single Yarrowia lipolytical fungal protoplasts

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sarah Williams

Single-cell metabolomics of bioproduction organisms such as Yarrowia lipolytica could significantly enhance our understanding of functional heterogeneity, yet remains a challenge due to limited input…

An automated droplet freezing assay system to study ice formation activities of soil and biogenic particles at warm temperatures

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nurun Nahar Lata

Ice nucleation (IN) efficiency of soil and biogenic particles at warmer temperatures (> -15° C) are least understood leading to poor representation of IN in models. We propose to build an…

Spatially-resolved proteome mapping of senescent cells and their tissue microenvironment at single-cell resolution

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Cellular senescence is a permanent state of cell cycle arrest induced by many different stresses. Although senescent cells (SNCs) have been demonstrated with beneficial roles in normal physiological…

High throughput single cell multiomics platform

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

In this collaborative project, PNNL and Scienion will co-develop an integrated microfluidic technology to co-measure the transcriptome and proteome in single cells. The technology will enable us to…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to comprehensively characterize human tumor samples provided by the National Cancer Institute (NCI), and to integrate the…

Integrated multi-omics analysis of multiple myeloma

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

In this project, integrated quantitative global proteomics and phosphoproteomics analysis of multiple myeloma cases (unsorted and sorted cell types) will be performed using multiplexed tandem mass…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Smith

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Undiagnosed Diseases Network Metabolomics Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

A state of the art metabolomics core will be a vital part of the Undiagnosed Diseases Network (UDN). However, in order for the UDN to realize it’s the full potential it is essential that the…

Undiagnosed Diseases Network Metabolomics Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

A state of the art metabolomics core will be a vital part of the Undiagnosed Diseases Network (UDN). However, in order for the UDN to realize it’s the full potential it is essential that the…

Integrated multi-omics analysis of multiple myeloma

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

In this project, integrated quantitative global proteomics and phosphoproteomics analysis of multiple myeloma cases (unsorted and sorted cell types) will be performed using multiplexed tandem mass…

Next generation microfluidics platform for high throughput single cell proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

The project aims to improve both sensitivity and throughput in single cell proteomics through the development of a microfluidic platform that leverages current technology. A hybrid picowell/nanowell…

SyPro Poplar: Improving Poplar Biomass Production under Abiotic Stress Conditions: an
Integrated Omics, Bioinformatics, Synthetic Biology and Genetic Engineering Approach

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

To engineer bioenergy crops that can grow in dry, marginal lands, this project will introduce into poplar trees tolerance to different environmental stresses such as water deficit, high soil salinity…

Engineering Synthetic Anaerobic Consortia Inspired by the Rumen for Biomass Breakdown and Conversion

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

Lignocellulosic plant biomass is a widely-abundant renewable resource that can be harnessed for value-added production of fuels & chemicals. While microbes have been engineered to breakdown…

Projects