Technology development in support of NCRR
EMSL Project ID
20496
Abstract
The Pacific Northwest National Laboratory (PNNL) has built a facility that already stands at the forefront of proteomic research. Moreover, the Center is uniquely poised for future developments. We plan to leverage the existing facilities and capabilities, and to extend the technology and expertise, by establishing a P41 Research Resource for the NIH-supported biomedical research community. We will develop new proteomic technologies for collaborative research and provide proteomics services and technology dissemination and training across the spectrum of proteomic methods, instrumentation and informatics areas. The facility will: (1) develop, integrate, and apply new analytical technologies; (2) develop and deploy the requisite, sophisticated computational and informatics tools for rapid, quantitative, and comprehensive proteomic measurements required for biomedical research; and (3) disseminate and propagate these technologies.
Project Details
Start Date
2006-06-13
End Date
2007-10-01
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Hamid AM, YM Ibrahim, VBS Garimella, IK Webb, L Deng, TC Chen, GA Anderson, SA Prost, RV Norheim, AV Tolmachev, and RD Smith. 2015. "Characterization of Traveling Wave Ion Mobility Separations in Structures for Lossless Ion Manipulations." Analytical Chemistry 87(22):11301-11308. doi:10.1021/acs.analchem.5b02481
Ioan Marginean, Ryan T. Kelly, Jason S. Page, Keqi Tang, Richard D. Smith, Electrospray characteristic curves: In pursuit of improved performance in the nanoflow regime, Anal. Chem., 2007, 79, 8030-8036.
Kim J, BC Kim, D Lopez-Ferrer, K Petritis, and RD Smith. 2010. "Nanobiocatalysis for protein digestion in proteomic analysis ." Proteomics 10(4 SP ISS):687-699.
Mabrouki RB, RT Kelly, DC Prior, AA Shvartsburg, K Tang, and RD Smith. 2009. "Improving FAIMS Sensitivity using a Planar Geometry with Slit Interfaces." Journal of the American Society for Mass Spectrometry 20(9):1768-1774. doi:10.1016/j.jasms.2009.05.019
Marginean I, RT Kelly, DC Prior, BL Lamarche, K Tang, and RD Smith. 2008. "Analytical Characterization of the Electrospray Ion Source in the Nanoflow Regime." Analytical Chemistry 80(17):6573-6579. doi:10.1021/ac800683s
Marginean I, RT Kelly, RJ Moore, DC Prior, BL Lamarche, K Tang, and RD Smith. 2009. "Selection of the Optimum Electrospray Voltage for Gradient Elution LC-MS Measurements." Journal of the American Society for Mass Spectrometry 20(4):682-688.
Petyuk VA, W Qian, RD Smith, and DJ Smith. 2010. "Mapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry." Methods 50(2):77-84.
Rasmussen A, DL Diamond, JE McDermott, X Gao, TO Metz, MM Matzke, V Carter, S Belisle, MJ Korth, KM Waters, RD Smith, and MG Katze. 2011. "Systems Virology Identifies a Mitochondrial Fatty Acid Oxidation Enyzme, Dodecenoyl Coenzyme A Delta Isomerase, Required for Hepatitis C Virus Replication and Likely Pathogenesis." Journal of Virology 85(22):11646-11654. doi:10.1128/JVI.05605-11
Shen Y, KK Hixson, N Tolic, DG Camp, II, SO Purvine, RJ Moore, and RD Smith. 2008. "Mass spectrometry analysis of proteome-wide proteolytic post-translational degradation of proteins." Analytical Chemistry 80(15):5819-5828. doi:10.1021/ac800077w
Shen Y, T Liu, N Tolic, BO Petritis, R Zhao, RJ Moore, SO Purvine, DG Camp, II, and RD Smith. 2010. "A strategy for degradomic-peptidomic analysis of the human blood plasma ." Journal of Proteome Research 9(5):2339-2346. doi:10.1021/pr901083m
Shvartsburg AA, DC Prior, K Tang, and RD Smith. 2010. "High-Resolution Differential Ion Mobility Separations Using Planar Analyzers at Elevated Dispersion Fields." Analytical Chemistry 82(18):7649-7655. doi:10.1021/ac101413k
Shvartsburg AA, K Tang, and RD Smith. 2010. "Differential Ion Mobility Separations of Peptides with Resolving Power Exceeding 50." Analytical Chemistry 82(1):32-35. doi:10.1021/ac902133n