EDRN Biomarker Reference and Resource Center with the Department of Energy
EMSL Project ID
43796
Abstract
The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous detection of ~50 prostate cancer biomarkers in tissue and body fluids; to conduct analytical and clinical validation studies, in collaboration with EDRN investigators; to estimate the utility of the developed assay(s) for early detection and diagnosis of prostate cancer; and to develop identical multiplex quantitative detection of biomarkers in additional cancers, pancreas and breast.
Project Details
Start Date
2011-04-18
End Date
2014-04-27
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Antibody-independent targeted quantification of TMPRSS2-ERG fusion protein products in prostate cancer
He J, X Sun, T Shi, AA Schepmoes, TL Fillmore, VA Petyuk, F Xie, R Zhao, MA Gritsenko, F Yang, N Kitabayashi, SS Chae, M Rubin, J Siddiqui, J Wei, AM Chinnaiyan, W Qian, RD Smith, J Kagan, S Srivastava, KD Rodland, T Liu, and DG Camp, II. 2014. "Antibody-independent Targeted Quantification of TMPRSS2-ERG Fusion Protein Products in Prostate Cancer." Molecular Oncology. doi:10.1016/j.molonc.2014.02.004
Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis
Shi T, TL Fillmore, Y Gao, R Zhao, J He, AA Schepmoes, CD Nicora, C Wu, JL Chambers, RJ Moore, J Kagan, S Srivastava, KD Rodland, T Liu, DG Camp, II, RD Smith, and W Qian. 2013. "Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis." Analytical Chemistry 85(19):9196-9203. doi:10.1021/ac402105s
Targeted Quantification of Low ng/mL Level Proteins in Human Serum without Immunoaffinity Depletion
Targeted quantification of low ng/mL level proteins in human serum without immunoaffinity depletion.
Shi T, Sun X, Gao Y, Fillmore TL, Schepmoes AA, Zhao R, He J, Moore RJ, Kagan J, Rodland KD, Liu T, Liu AY, Smith RD, Tang K, Camp DG 2nd, Qian WJ.
J Proteome Res. 2013 Jul 5;12(7):3353-61. doi: 10.1021/pr400178v. Epub 2013 Jun 13.
PMID: 23763644
Shi T, Sun X, Gao Y, Fillmore TL, Schepmoes AA, Zhao R, He J, Moore RJ, Kagan J, Rodland KD, Liu T, Liu AY, Smith RD, Tang K, Camp DG 2nd, Qian WJ.
J Proteome Res. 2013 Jul 5;12(7):3353-61. doi: 10.1021/pr400178v. Epub 2013 Jun 13.
PMID: 23763644
Root protein profile changes induced by Al exposure in two rice cultivars differing in Al tolerance
Liu T, M Hossain, AA Schepmoes, TL Fillmore, LJ Sokoll, SR Kronewitter, G Izmirlian, T Shi, W Qian, R Leach, IM Thompson, DW Chan, RD Smith, J Kagan, S Srinivastava, KD Rodland, and DG Camp, II. 2012. "Analysis of Serum Total and Free PSA Using Immunoaffinity Depletion Coupled to SRM: Correlation with Clinical Immunoassay Tests." Journal of Proteomics. doi:doi: 10.1016/j.jprot.2012.09.035 [In Press]