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EDRN Biomarker Reference and Resource Center with the Department of Energy


EMSL Project ID
43796

Abstract

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous detection of ~50 prostate cancer biomarkers in tissue and body fluids; to conduct analytical and clinical validation studies, in collaboration with EDRN investigators; to estimate the utility of the developed assay(s) for early detection and diagnosis of prostate cancer; and to develop identical multiplex quantitative detection of biomarkers in additional cancers, pancreas and breast.

Project Details

Start Date
2011-04-18
End Date
2014-04-27
Status
Closed

Team

Principal Investigator

Tao Liu
Institution
Pacific Northwest National Laboratory

Team Members

Thomas Fillmore
Institution
Environmental Molecular Sciences Laboratory

Tujin Shi
Institution
Pacific Northwest National Laboratory

Fang Xie
Institution
Pacific Northwest National Laboratory

Mahmud Hossain
Institution
Pacific Northwest National Laboratory

Weijun Qian
Institution
Pacific Northwest National Laboratory

David Camp
Institution
Pacific Northwest National Laboratory

Karin Rodland
Institution
Pacific Northwest National Laboratory

Related Publications

A Highly Sensitive Targeted Mass Spectrometric Assay for Quantification of AGR2 Protein in Human Urine and Serum. Shi T, Gao Y, Quek SI, Fillmore TL, Nicora CD, Su D, Zhao R, Kagan J, Srivastava S, Rodland KD, Liu T, Smith RD, Chan DW, Camp DG 2nd, Liu AY, Qian WJ. J Proteome Res. 2013 Dec 3. [Epub ahead of print] PMID: 24251762
He J, X Sun, T Shi, AA Schepmoes, TL Fillmore, VA Petyuk, F Xie, R Zhao, MA Gritsenko, F Yang, N Kitabayashi, SS Chae, M Rubin, J Siddiqui, J Wei, AM Chinnaiyan, W Qian, RD Smith, J Kagan, S Srivastava, KD Rodland, T Liu, and DG Camp, II. 2014. "Antibody-independent Targeted Quantification of TMPRSS2-ERG Fusion Protein Products in Prostate Cancer." Molecular Oncology. doi:10.1016/j.molonc.2014.02.004
Liu T, M Hossain, AA Schepmoes, TL Fillmore, LJ Sokoll, SR Kronewitter, G Izmirlian, T Shi, W Qian, R Leach, IM Thompson, DW Chan, RD Smith, J Kagan, S Srinivastava, KD Rodland, and DG Camp, II. 2012. "Analysis of Serum Total and Free PSA Using Immunoaffinity Depletion Coupled to SRM: Correlation with Clinical Immunoassay Tests." Journal of Proteomics. doi:doi: 10.1016/j.jprot.2012.09.035 [In Press]
Shi T, D Su, T Liu, K Tang, DG Camp, II, W Qian, and RD Smith. 2012. "Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics." Proteomics 12(8):1074-1092. doi:10.1002/pmic.201100436
Shi T, TL Fillmore, X Sun, R Zhao, AA Schepmoes, M Hossain, F Xie, S Wu, JS Kim, NJ Jones, RJ Moore, L Pasa-Tolic, J Kagan, KD Rodland, T Liu, K Tang, DG Camp, II, RD Smith, and W Qian. 2012. "Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum." Proceedings of the National Academy of Sciences of the United States of America 109(38):15395-15400. doi:10.1073/pnas.1204366109
Shi T, TL Fillmore, Y Gao, R Zhao, J He, AA Schepmoes, CD Nicora, C Wu, JL Chambers, RJ Moore, J Kagan, S Srivastava, KD Rodland, T Liu, DG Camp, II, RD Smith, and W Qian. 2013. "Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis." Analytical Chemistry 85(19):9196-9203. doi:10.1021/ac402105s
Targeted quantification of low ng/mL level proteins in human serum without immunoaffinity depletion. Shi T, Sun X, Gao Y, Fillmore TL, Schepmoes AA, Zhao R, He J, Moore RJ, Kagan J, Rodland KD, Liu T, Liu AY, Smith RD, Tang K, Camp DG 2nd, Qian WJ. J Proteome Res. 2013 Jul 5;12(7):3353-61. doi: 10.1021/pr400178v. Epub 2013 Jun 13. PMID: 23763644