High Resolution and Mass Accuracy Capability (HRMAC) Development Project
EMSL Project ID
49209
Abstract
Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross platform baseline performance measurements. Fourier transform ion cyclotron mass spectrometry (FTICR-MS), the highest performance mass spectrometry technique, is uniquely valued as a research tool for analytical applications requiring the highest possible resolution and mass measurement accuracy. No known alternatives exist for achieving the highest levels of resolution and mass accuracy as obtainable with FTICR-MS. The design for an HRMAC system will comprise the design and construction of an advanced FTICR-MS system. A highfield, wide-horizontal bore magnet is critical to the design. While not previously constructed, two different vendors currently have demonstrated high probability of successfully delivering a magnet substantially exceeding homogeneity and field strength of current FTICR magnets. These designs are based on demonstrated manufacturing expertise using advanced superconducting magnet technology. As the design plan places priority on long lead time components that constrain the critical path of the project, awarding the acquisition contract for this magnet is EMSL’s highest priority. As the largest and longest lead time component of the HRMAC system, the magnet’s construction and delivery is the primary driver of the project schedule. The HRMAC system will provide a high-demand capability at EMSL that will address some of the most challenging compositional and structural analysis questions underlying complex chemical, biological, energy, and environmental problems and phenomena, including: - Biofuel and new energy sources development. - Efficient methods for carbon capture and sequestration. - Understanding biology of complex systems (e.g., microbial communities). - The transport and fate of key metals, metabolites, and toxic contaminants, including radionuclides and trace organics in the subsurface. A particularly important driver for this new capability is enhancing and extending EMSL’s leadership role in proteomics at both the peptide and intact protein level.
Project Details
Start Date
2016-04-27
End Date
2016-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Gargano A., J.B. Shaw, M. Zhou, C.S. Wilkins, T.L. Fillmore, R.J. Moore, and G.W. Somsen, et al. 2018. "Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS." Journal of Proteome Research 17, no. 11:3791-3800. PNNL-SA-138847. doi:10.1021/acs.jproteome.8b00458
Shaw JB, TY Lin, FE Leach, III, AV Tolmachev, N Tolic, EW Robinson, DW Koppenaal, and L Pasa Tolic. 2016. "21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer Greatly Expands Mass Spectrometry Toolbox." Journal of the American Society for Mass Spectrometry 27(12):1929-1936. doi:10.1007/s13361-016-1507-9