Projects

Towards a systems model of circadian control of gene function to regulate metabolic phenotype and cellulase production in Neurospora crassa

Lead Institution

Rensselaer Polytechnic Institute

Principal Investigator

Jennifer Hurley

Project type

Large-Scale EMSL Research

Biofuel production using cellulases extracted from fungi has the potential to be a major resource to replace fossil fuels, and regulating the synthesis of these and other enzymes is crucial for the…

Exploring the native cell wall nanoarchitecture of engineered bioenergy crops via multi-dimensional solid-state NMR.

Lead Institution

University of Adelaide

Principal Investigator

Jennifer Mortimer

Project type

Large-Scale EMSL Research

Carbon dioxide from the atmosphere, an inorganic carbon source, is fixed by plants via photosynthesis to form the polysaccharides and lignin that constitute the plant cell wall. The plant cell wall,…

Selective Sensors for High Spatiotemporal Imaging of Small Molecule Targets in Metabolic Pathways of Plants and Cells involved in Bio-energy Processes

Lead Institution

University of Texas at Austin

Principal Investigator

Yi Lu

Project type

Large-Scale EMSL Research

As a member of the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), a DOE Bioenergy Center at the University of Illinois at Urbana-Champaign, we aim to develop selective sensors to…

Cryo-FIB and High-Vacuum Cryo-Transfer for Atom Probe Tomography Analysis of Organic Molecular Materials

Lead Institution

Colorado School of Mines

Principal Investigator

Jeramy Zimmerman

Project type

Large-Scale EMSL Research

The morphology of mixed molecular solids impacts macroscopic properties of the material (e.g. conductivity). Our group has developed atom probe tomography (APT) analysis methods for organic small…

Identifying dynamic sources and transformations of dissolved organic matter in coastal watersheds exposed to extreme flooding

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nicholas Ward

Project type

Large-Scale EMSL Research

The primary objective of this proposal is to improve mechanistic understanding of the hydrobiogeochemical processes governing the cycling of organic matter (OM) across the land-to-sea continuum…

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

Rhodobacter sphaeroides is an alpha-proteobacterium which can remodel its cell envelope under low-oxygen or anaerobic conditions, by increasing its lipid content and forming intracytoplasmic membrane…

The Diurnal Redox Proteome of Chlamydomonas

Lead Institution

Michigan State University

Principal Investigator

Daniela Strenkert

Project type

Large-Scale EMSL Research

Nearly all organisms exhibit diurnal metabolic cycles, especially phototrophs, whose metabolism is strictly dependent on light. Transcriptomics experiments in cyanobacteria, algae and plants have…

Chemical Imaging and Ice Nucleation Properties of Individual Arctic Atmospheric Particles

Lead Institution

University of Michigan

Principal Investigator

Kerri Pratt

Project type

Large-Scale EMSL Research

The proposed project will address critical gaps in our understanding of the Arctic aerosol sources, chemical composition, and ice nucleation properties through chemical imaging and ice nucleation…

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

Large-Scale EMSL Research

Determining in situ metabolic processes and interactions among diverse assemblages of microorganisms remains a critical challenge in microbial ecology. Consequently, identifying novel genes and…

Environmental Photochemical Catalysis and Microphysical Properties of Fe-Organic Aerosols

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Project type

Large-Scale EMSL Research

The intent of this collaborative research is to investigate photo-catalytic effects of atmospheric aging on the chemical composition and the microphysical properties of secondary organic aerosol (SOA…

Connecting Structure and Function in Anaerobic Fungal Cellulosomes

Lead Institution

University of California, Santa Barbara

Principal Investigator

Michelle O'Malley

Project type

Large-Scale EMSL Research

This proposal seeks use of EMSL resources to characterize the unique, multi-enzyme cellulosomes made by anaerobic gut fungi that are found within large herbivores. Cellulosomes enable anaerobic…

Uncovering the phosphoproteome of arbuscular mycorrhizal roots

Lead Institution

Boyce Thompson Institute for Plant Research

Principal Investigator

Maria Harrison

Project type

Large-Scale EMSL Research

During the endosymbiotic association of plants and arbuscular mycorrhizal (AM) fungi, the fungus inhabits the root cortical cells where it delivers mineral nutrients to its plant host, and in return…

Modeling Solvent Effects in Mixed-Solvent Environments for Improved Biomass Conversion

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Reid Van Lehn

Project type

Large-Scale EMSL Research

The conversion of lignocellulosic biomass is a promising method to produce biofuels and chemicals for further microbial conversion from a renewable carbon resource via liquid-phase, acid-catalyzed…

Integrated experimental and modeling study to investigate vertical profile of atmospheric particles via tethered balloon system

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Fan Mei

Project type

Large-Scale EMSL Research

We propose a combined experimental and modeling study to characterize the vertical profile of atmospheric aerosol collected via a tethered balloon system using multi-modal micro-spectroscopy…

Coupling spectral techniques; Molecular characterization of dissolved organic matter across the major soils and ecosystems of the United States using fluorescence spectroscopy and Fourier transform ion cyclotron resonance mass spectroscopy

Lead Institution

University of Colorado at Boulder

Principal Investigator

Michael Sanclements

Project type

Large-Scale EMSL Research

This User proposal will facilitate the completion of data analysis central to the broader research effort investigating soil organic matter (SOM) stabilization and vulnerability using a multi-scale…

Computational Studies of Nanoparticle Formation and Bulk Properties for Biomineralization

Lead Institution

University of Alabama

Principal Investigator

David Dixon

Project type

Large-Scale EMSL Research

There is a critical need to develop an understanding of nanoparticle and mineral formation in systems that contain H2O, metal ions and CO2 as carbonation and hydrolysis reactions are important for…

Atomic Resolution of the Structure and Fungal Degradation of Wood Secondary Cell Walls

Lead Institution

Michigan State University

Principal Investigator

Tuo Wang

Project type

Large-Scale EMSL Research

The proposed research aims at obtaining atomic-level information to construct four structural models that reflect the supramolecular architecture of plant secondary cell walls in hardwoods and…

Aerosol-Ice Formation Closure Study Applying Ambient Aerosol Particles

Lead Institution

State University of New York at Stony Brook

Principal Investigator

Daniel Knopf

Project type

Large-Scale EMSL Research

Atmospheric ice nucleation represents one of the least understood atmospheric processes, hampering prediction of cloud processes and climate. Whereas the aerosol community has widely conducted…

Structural characterization of nodule-specific cysteine-rich antifungal peptides and their phospholipid receptors for elucidation of their modes of action

Lead Institution

Donald Danforth Plant Science Center

Principal Investigator

Dilip Shah

Project type

Large-Scale EMSL Research

Antimicrobial peptides (AMPs) are a major component of innate immunity against infectious diseases in animals and plants [2]. A feature prominent in many families of AMPs, such as defensins (Figure 1…

Coupling Measurements and Modeling to Understand the Lifecycle of Secondary Organic Aerosol over Local to Regional Scales

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jerome Fast

Project type

Large-Scale EMSL Research

Our hypothesis is that a better understanding of fast-reacting processes, such as the chemistry of volatile organic compounds associated with isoprene having a lifetime of ~minutes, is needed to make…