Kyle Pomraning

Agile Biofoundry FY24-25

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nathalie Munoz Munoz

This proposed EMSL Staff time proposal assists the DOE-EERE-BETO supported PNNL project: FY24-25 seven-national laboratory Agile BioFoundry (https://agilebiofoundry.org/). PNNL’s effort for this…

Microbial community engineering to reduce carbon and nitrogen footprints in biomanufacturing

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kyle Pomraning

Conventional bioplatforms for production of renewable fuels and chemicals often represent monocultures of heterotrophic microorganisms (e.g. fungi) that require feeding with organic carbon (e.g…

Unraveling New Pathways for Carbon Capture to Enhance Lipid-Biomass Feedstocks

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Yarrowia lipolytica for biotechnology and synthetic biology

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Yarrowia lipolytica for biotechnology and synthetic biology

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Erin Bredeweg

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics/metabolomics to establish baseline cell biological and metabolite data on DNA agility in eukaryotic organisms, and…

Microbial community engineering to reduce carbon and nitrogen footprints in biomanufacturing

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kyle Pomraning

Conventional bioplatforms for production of renewable fuels and chemicals often represent monocultures of heterotrophic microorganisms (e.g. fungi) that require feeding with organic carbon (e.g…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Decreasing the amount of material required for gross biological carbon and nitrogen flux measurements by more than 12 orders of magnitude using ion implantation isotope dilution and stable isotope measurements by NanoSIMS

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

John Cliff

Advances in mass spectrometry coupled to stable isotope probing techniques have profoundly influenced our understanding of process level biology and biogeochemistry. When stable isotope compounds and…

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