Natalie Sadler | Environmental Molecular Sciences Laboratory

Investigating the Role of Drought-Impacted Root Exudates on Rhizosphere Microbial Metabolism and Viral Dynamics for Improving Tall Wheatgrass Drought Tolerance

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

Project type

Large-Scale EMSL Research

The rhizosphere is the center of interactions between roots, soil, and microbes, where chemical exchanges are key drivers of microbial composition, plant productivity, and resilience to abiotic…

Linking multi-organism-environment interactions across lab and field scales to estimate viral contributions to soil C cycling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Amy Zimmerman

Project type

FICUS Research

Interactions between soil viruses and their microbial hosts can significantly impact microbial population dynamics, metabolism, and evolution, with cascading effects on soil carbon (C)…

Revealing the secretome and enriching the function of newly established hyperthermophilic microbial communities with superior cellulolytic and hemicellulolytic activities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Pavlo Bohutskyi

Project type

Exploratory Research

Vast amount of organic waste materials is generated in U.S. These carbon-containing waste streams represent a potentially valuable but underutilized feedstock for production of carbon-neutral…

TEAMS - Trial Ecosystems for the Advancement of Microbiome Science

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

A primary goal of the TEAMs project is to optimize the Soil EcoFAB devices to accommodate goals of the soil microbiome scientific community. We have made significant progress advancing the Soil…

Determination of the Molecular Architecture and Temporal Dynamics that Drive Microbial Lignocellulose Degradation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Song Feng

Project type

Large-Scale EMSL Research

In order to link phenotypic outcomes to molecular determinants, we have developed an integrated functional profiling platform, PhenoProfiling, that pairs activity-based probes (ABPs) with…

Disentangling the role of microbial interactions and spatial structure in soil organic matter decomposition

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

Project type

Exploratory Research

The study of the soil microbiome is critical to understanding the ecological functions carried out by soil microorganisms, particularly with respect to the cycling of carbon (C) and nitrogen (N)…

Regulatory dynamics of microbial communities

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

H Wiley

Project type

Large-Scale EMSL Research

This is a proposal to conduct work in EMSL in support of the Foundational Scientific Focus Area (FSFA) research program at Pacific Northwest National Laboratory. This large-scale research program…

Identification of Functional Proteins Relevant to Bioenergy and Disease Pathology by Multiplexed Activity-Based Protein Profiling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

Develop multiplexed activity-based protein profiling and apply to identification of P450s in Mycobacterium tuberculosis and biomarkers in Aspergillus fumigatus. Additionally the technology will be…

Combining Proteomic Technologies to Create a Platform for Spatiotemporal Enzyme Activity Profiling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Susan Wiedner

There is a need to investigate the functional activity of enzymes on the subcellular level to increase the understanding of a multitude of biological processes and protein interactions. The…

Genome-Enabled Studies of Photosynthetic Microorganisms for Bioenergy Applications - Chemical Protein Profiling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

As part of PNNL's Scientific Focus Area "Genome-Enabled Studies of Photosynthetic Microorganisms for Bioenergy Applications" this project is applying and developing click-chemistry…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Activity-Based Protein Profiling of Enzyme Ontogeny: Metabolism in Sensitive Life Stages

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Corley

Metabolism is a key step in the activation of many procarcinogens, including the polyaromatic hydrocarbon (PAH) dibenzo[def,p]chrysene (DBC). DBC is a highly potent carcinogenic PAH, capable of…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Decomposers in Transition

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

The overarching objective of the research is to develop a scalable approach for understanding how soil decomposer community dynamics are affected by spatial heterogeneity and climate change (drought)…

Multiplexed Probing for Functional Capacity Measurements in Complex and Limited-Size Samples

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

The TIP LDRD program at PNNL has supported the development, application and patenting of novel strategies to functionalize activity-based chemical probes to glass surfaces for making measurements of…

Identification of plant host and microbe model system for study of rhizophagy cycle

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

Rhizophagy (“root eating”) is a process by which plants actively uptake soil microbes into their roots and use oxidative degradation to extract micronutrients from the cells. This phenomenon has been…

Profiling protease activities in sorghum under drought stress and recovery

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will investigate proteases as signatures of plant drought stress by using in vivo activity-based protein profiling to identify and evaluate the contributions of distinct serine and cysteine…

Spatially resolved chemical proteomics for linking enzymatic activity to omics phenotyping in heterogeneous microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will connect chemical probes and nanoPOTS to rapidly identify microbial species and essential protein functions in structured microbial communities. We will use aggregation induced emission (AIE)…

Projects